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- rabbit polyclonal IgG, 200 µg/ml
- epitope corresponding to amino acids 303-478 mapping at the C-terminus of CAMKIIα of mouse origin
- recommended for detection of all CaMKII subunits of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
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CaMKII Background Information
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated å, ∫, © and ∂, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177.
| CaMKII (M-176) Product Citations |
See how others have used CaMKII (M-176): sc-9035 antibody and or CaMKII (M-176) antibody conjugates.
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CaMKII (M-176)
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Western blot detection of CaMKII phosphorylation in mouse brain extracts. Blots were probed with CaMKII (M-176): sc-9035 (A) and p-CaMKIIα (Thr 286)-R: sc-12886-R (B,C). In B and C, the antibody was preincubated with cognate non-phosphorylated or phosphorylated peptide, respectively. CaMKII (M-176): sc-9035. Western blot analysis of CaMKII expression in rat brain extract.
CaMKII (M-176): sc-9035. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic staining.
CaMKII (M-176): sc-9035. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
CaMKII (M-176): sc-9035. Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing cytoplasmic and nuclear staining of respiratory epithelial cells and glandular cells.
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