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home » Transcription Regulators » RNA Polymerase Antibody / RNA Polymerase Antibodies » Pol II (C-21): sc-900

Pol II (C-21) Antibody: sc-900

  |  Datasheet
  • rabbit polyclonal IgG, 200 µg/ml
  • epitope mapping within the tandem repeat domain of the large subunit of RNA polymerase II (RPB1) of mouse origin
  • recommended for detection of Pol II of mouse, rat and human origin by WB, IP, IF and ELISA; may cross-react with NF-1 protein; also reactive with additional species, including canine and bovine
  • blocking peptide, sc-900 P
  • agarose conjugate for IP studies, sc-900 AC, 500 µg/0.25 ml agarose
  • TransCruz reagent for ChIP application, sc-900 X, 200 µg/0.1 ml
 
Additional RNA Polymerase Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   ChIP  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human POLR2A 5430 17p13.1 NM_000937 P24928
 180660
Mouse Polr2a 20020 11 B3 P08775
 N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
Pol II (C-21) sc-900 200 µg/ml $279
Pol II (C-21) AC sc-900 AC 500µg/ml, 25%ag $366
Pol II (C-21) P sc-900 P
(peptide)
 100 µg/0.5 ml $61
Pol II (C-21) X sc-900 X 200 µg/0.1 ml $279
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
A-431 nuclear extract sc-2122 1000 µg $143
A-673 nuclear extract sc-2128 1000 µg $143
U-2 OS Cell Lysate sc-2295 500 µg/200 µl $104
HeLa nuclear extract sc-2120 1000 µg $143
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
PC-12 Cell Lysate sc-2250 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104

Pol II Background Information
RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Pol II (C-21) Product Citations
See how others have used Pol II (C-21): sc-900 antibody and or Pol II (C-21) antibody conjugates.


26 total citations
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Pol II (C-21)
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sc-900
Western blot analysis of Pol II expression in A-431 (A,D), AT-3 (B,E) and A-673 (C,F) nuclear extracts. Antibodies tested include Pol II (C-21): sc-900 (A-C) and Pol II (N-20): sc-899 (D-F).
sc-900
ChIP analysis of recruitment of basal transcription factors Pol II and TBP to the IL-2Rα (CD25) promoter in vivo. Antibodies tested include Pol II (C-21): sc-900 and TFIID (TBP) (SI-1): sc-273. CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag-/- or WT thymocytes. Data kindly provided by J. Imbert and reproduced from Yeh, J.H., et al. 2002. Nucleic Acids Res. 30: 1944-1951, with permission from Oxford University Press.
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