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USF-1 Antibody (H-86): sc-8983

 |  Datasheet

(Based on data analysis)

  • USF-1 Antibody (H-86) is a rabbit polyclonal IgG provided at 200 µg/ml
  • epitope corresponding to amino acids 75-160 mapping within an internal region of USF-1 of human origin
  • recommended for detection of USF-1 of mouse, rat and human origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3739)- and FITC (sc-2012)-conjugated goat anti-rabbit IgG; also reactive with additional species, including equine, canine, bovine and porcine
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-8983 X, 200 µg/0.1 ml
  • See 26 citations for USF-1 Antibody (H-86)
 
Full-length USF-1 protein sequence with immunogen highlighted:
MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

See additional USF Antibodies including USF-2, USF-1 and USF.

Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   FCM   Gel Shift   ChIP   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
USF-1 Antibody (H-86) sc-8983 200 µg/ml $279
USF-1 Antibody (H-86) X sc-8983 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
USF-1 siRNA (h) sc-36783 10 µM $258
USF-1 siRNA (m) sc-36784 10 µM $258
USF-1 (h)-PR sc-36783-PR 10 µM, 20 µl $23
USF-1 (m)-PR sc-36784-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
USF-1 shRNA Plasmid (h) sc-36783-SH 20 µg $520
USF-1 shRNA Plasmid (m) sc-36784-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
USF-1 shRNA (h) Lentiviral Particles sc-36783-V 200 µl $625
USF-1 shRNA (m) Lentiviral Particles sc-36784-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NIH/3T3 nuclear extract sc-2138 250 µg/0.05 ml $143
Jurkat nuclear extract sc-2132 250 µg/0.05 ml $143
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
USF-1 (m): 293T Lysate sc-124487 100 µg/200 µl $205
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human USF1 7391 1q23.3 NM_007122, NM_207005 P22415
602491
Mouse Usf1 22278 1 H3 Q61069
N/A
 


The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of (USF-1) and (USF-2) polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an upstream stimulatory factor that binds the core sequence CACGTG in the adenovirus late promoter. These findings, together with the demonstration of cooperative interaction between USF and the initiator-binding protein, TFII-I, raises the possibility of a more general involvement of USF in transcriptional regulation. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.

USF-1 Antibody (H-86) Product Citations
See how others have used USF-1 Antibody (H-86) and or USF-1 Antibody (H-86) conjugates.


26 total citations
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USF-1 Antibody (H-86) Data
Click on image to enlarge
USF-1 (H-86): sc-8983. Western blot analysis of USF-1 expression in Jurkat nuclear extract.
USF-1 (H-86): sc-8983. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with USF-1 (H-86), followed by FITC-conjugated goat anti-rabbit IgG: sc-2012. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888.
USF-1 siRNA (h): sc-36783. Immunofluorescence staining of methanol-fixed, control HeLa (A) and USF-1 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with USF-1 (H-86): sc-8983.
USF-1 (H-86): sc-8983. Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing nuclear staining of surface epithelial cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
USF-1 (H-86): sc-8983. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with USF-1 (H-86), followed by PE-conjugated goat anti-rabbit IgG: sc-3739. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888.
USF-1 (H-86): sc-8983. Western blot analysis of USF-1 expression in non-transfected: sc-117752 (A) and mouse USF-1 transfected: sc-124487 (B) 293T whole cell lysates.
USF-1 (H-86): sc-8983. Western blot analysis of USF-1 expression in non-transfected: sc-117752 (A) and mouse USF-1 transfected: sc-124487 (B) 293T whole cell lysates.
USF-1 (H-86): sc-8983. Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nuclear staining of urothelial cells.
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