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home » Cell Cycle Proteins » Chk Antibody / Chk Antibodies » Chk2 (C-18): sc-8813

Chk2 (C-18) Antibody: sc-8813

  |  Datasheet
  • goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-8813-R
  • epitope mapping near the C-terminus of Chk2 of human origin
  • recommended for detection of Chk2 of human origin by WB, IP, IF, IHC(P) and ELISA
  • blocking peptide, sc-8813 P
  • agarose conjugate for IP studies, sc-8813 AC, 500 µg/0.25 ml agarose
 
Additional Chk Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human CHEK2 11200 22q12.1 NM_001005735, NM_007194, NM_145862 O96017
 609265
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
Chk2 (C-18) sc-8813 200 µg/ml $279
Chk2 (C-18) AC sc-8813 AC 500µg/ml, 25%ag $366
Chk2 (C-18) P sc-8813 P
(peptide)
 100 µg/0.5 ml $61
Chk2 (C-18)-R sc-8813-R 200 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Chk2 siRNA (h) sc-29271 10 µM $258
Chk2 (h)-PR sc-29271-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Chk2 shRNA Plasmid (h) sc-29271-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Chk2 shRNA (h) Lentiviral Particles sc-29271-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
HL-60 Whole Cell Lysate sc-2209 500 µg/200 µl $104
HuT 78 Whole Cell Lysate sc-2208 500 µg/200 µl $104
HeLa + heat shock Cell Lysate sc-2272 500 µg/200 µl $104
CCRF-CEM Cell Lysate sc-2225 500 µg/200 µl $104
Saos-2 Cell Lysate sc-2235 500 µg/200 µl $104

Chk2 Background Information
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.

Chk2 (C-18) Product Citations
See how others have used Chk2 (C-18): sc-8813 antibody and or Chk2 (C-18) antibody conjugates.


3 total citations
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Chk2 (C-18)
Click on image to enlarge
sc-8813
Chk2 (C-18): sc-8813. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor showing nuclear staining (A). Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining (B).
sc-8813
Chk2 (C-18): sc-8813. Western blot analysis of Chk2 expression in HeLa whole cell lysate.
sc-8813
Chk2 siRNA (h): sc-29271. Immunofluorescence staining of methanol-fixed, control HeLa (A) and Chk2 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with Chk2 (C-18)): sc-8813.
sc-8813
Chk2 (C-18): sc-8813. Western blot analysis of Chk2 expression in HeLa (A), CCRF-CEM (B) and Saos-2 (C) whole cell lysates.
sc-8813
Chk2 (C-18): sc-8813. Western blot analysis of Chk2 expression in HL-60 whole cell lysate.
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