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MKLP-1 Antibody (N-19): sc-867

 |  Datasheet

(Based on data analysis)

  • MKLP-1 Antibody (N-19) is a rabbit polyclonal IgG provided at 200 µg/ml
  • epitope mapping at the N-terminus of MKLP-1 of human origin
  • recommended for detection of MKLP-1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
  • blocking peptide, sc-867 P
  • agarose conjugate for IP studies, sc-867 AC, 500 µg/0.25 ml agarose
  • See 14 citations for MKLP-1 Antibody (N-19)
 

See additional MKLP-1 Antibodies.

Also see MKLP-1 Inhibitors for functional analysis of cellular responses to MKLP-1.


Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   siRNA  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
MKLP-1 Antibody (N-19) sc-867 200 µg/ml $279
MKLP-1 Antibody (N-19) AC sc-867 AC 500 µg/ml, 25% ag $366
MKLP-1 (N-19) P sc-867 P
(peptide)
100 µg/0.5 ml $61
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
MKLP-1 siRNA (h) sc-35936 10 µM $258
MKLP-1 siRNA (m) sc-37626 10 µM $258
MKLP-1 siRNA (r) sc-270014 10 µM $258
MKLP-1 (h)-PR sc-35936-PR 10 µM, 20 µl $23
MKLP-1 (m)-PR sc-37626-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
MKLP-1 shRNA Plasmid (h) sc-35936-SH 20 µg $520
MKLP-1 shRNA Plasmid (m) sc-37626-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
MKLP-1 shRNA (h) Lentiviral Particles sc-35936-V 200 µl $625
MKLP-1 shRNA (m) Lentiviral Particles sc-37626-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
K-562 nuclear extract sc-2130 250 µg/0.05 ml $143
MKLP-1 (h): 293T Lysate sc-113424 100 µg/200 µl $205
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human KIF23 9493 15q23 NM_004856, NM_138555 Q02241
605064
Mouse Kif23 71819 9 B NP_077207
N/A
 


The monoclonal antibody CHO1 detects a spindle antigen required for mitotic progression. Screening a HeLa cell cDNA expression library with this antibody has been shown to yield a cDNA predicted to encode a protein significantly related within its amino terminal half to the motor ends of members of the kinesin superfamily. Since this similarity does not extend further, it has been suggested that the CHO1 antigen, now designated MKLP-1 (mitotic kinesin-like protein-1), represents a novel kinesin. Sequence analysis has also been shown to predict that MKLP-1 possesses features typical of nuclear proteins. Immunocytological studies have demonstrated that MKLP-1 moves from the nucleus early in mitosis and then to the midbody after cytokinesis. MKLP-1 has been shown to bundle antiparallel microtubules in vitro and to move them at rates comparable to spindle elongation in vivo. A hamster homolog of MKLP-1, designated CHO1 antigen, has also been isolated. Although apparently functionally equivalent with respect to microtubule bundling activity, there are significant differences between the human and hamster proteins at their C-termini, possibly due to alternative splicing or the presence of more than one MKLP-1 locus.

MKLP-1 Antibody (N-19) Product Citations
See how others have used MKLP-1 Antibody (N-19) and or MKLP-1 Antibody (N-19) conjugates.


14 total citations
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MKLP-1 Antibody (N-19) Data
Click on image to enlarge
MKLP-1 (N-19): sc-867. Western blot analysis of MKLP-1 expression in K-562 whole cell lysate.
MKLP-1 (N-19): sc-867. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.
MKLP-1 (N-19) sc-867. Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing nuclear and cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
MKLP-1 (N-19): sc-867. Western blot analysis of MKLP-1 expression in non-transfected 293T: sc-117752 (A), human MKLP-1 transfected 293T: sc-113424 (B) and K-562 (C) whole cell lyastes.
MKLP-1 (N-19): sc-867. Western blot analysis of MKLP-1 expression in non-transfected: sc-117752 (A) and human MKLP-1 transfected: sc-116361 (B) 293T whole cell lysates.
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