Ac-Histone H3 (Lys9/14) Antibody sc-8655

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Ac-Histone H3 (Lys9/14) Antibody: sc-8655	\| Datasheet

goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-8655-R
epitope mapping near the N-terminus of Ac-Histone H3 of human origin
recommended for dectection of Histone H3 of mouse, rat, human, Drosphila, Xenopus and C. elegans origin acetylated at Lys 9 and Lys 14 by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
blocking peptide, sc-8655 P
Suitable for use as control antibody for Histone H3 (Ser 28): sc-24516.

Additional Histone Antibodies ...

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WB IP IF siRNA

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number

Human	H3F3A	3020	1q42.12	NM_002107	P84243	601128
Human	H3F3B	3021	17q25.1	NM_005324	P84243	601058
Human	HIST3H3	8290	1q42.13	NM_003493	Q16695	602820
Human	HIST1H3A	8350	6p22.1	NM_003529	P68431	602810
Human	HIST1H3D	8351	6p22.1	NM_003530	NP_003521	602811
Human	HIST1H3C	8352	6p22.1	NM_003531	P68431	602812
Human	HIST1H3E	8353	6p22.1	NM_003532	P68431	602813
Human	HIST1H3I	8354	6p22.1	NM_003533	P68431	602814
Human	HIST1H3G	8355	6p22.1	NM_003534	P68431	602815
Human	HIST1H3J	8356	6p22.1	NM_003535	P68431	602817
Human	HIST1H3H	8357	6p22.1	NM_003536	P68431	602818
Human	HIST1H3B	8358	6p22.1	NM_003537	P68431	602819
Human	HIST1H3F	8968	6p22.1	NM_021018	P68431	602816
Human	HIST2H3C	126961	1q21.2	NM_021059	Q71DI3	142780
Human	HIST2H3A	333932	1q21.2	NM_001005464	Q71DI3	n/a
Human	LOC440093	440093	12p11.21	NM_001013699	Q6NXT2	n/a
Human	LOC644914	644914	4q31.1	XM_930111	XP_935204	n/a
Human	HIST2H3D	653604	1q21.2	XM_497711	NP_001116847
Mouse	Hist2h3c1	15077	3 F2.1		P84228	N/A
Mouse	Hist2h3c2	97114	3 F2.1		P84228	N/A
Mouse	Hist1h3g	97908	13 A3.1		P68433	N/A
Mouse	Hist1h3f	260423	13 A3.1		P84228	N/A
Mouse	Hist1h3c	319148	13 A3.1		P84228	N/A
Mouse	Hist1h3d	319149	13 A3.1		P84228	N/A
Mouse	Hist1h3b	319150	13 A3.1		P84228	N/A
Mouse	Hist1h3e	319151	13 A3.1		P84228	N/A
Mouse	Hist1h3h	319152	13 A3.1		P68433	N/A
Mouse	Hist1h3i	319153	13 A3.1		P68433	N/A
Mouse	Hist2h3b	319154	3 F2.1		P84228	N/A
Mouse	Hist1h3a	360198	13 A3.1		P68433	N/A

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Ordering Information

Product Name	Catalog #	Unit	Price
Ac-Histone H3 (Lys9/14)	sc-8655	200 µg/ml	$279
Ac-Histone H3 (Lys 9/14) P	sc-8655 P (peptide)	100 µg/0.5 ml	$61
Ac-Histone H3 (Lys 9/14)-R	sc-8655-R	200 µg/ml	$279

WB Positive Control Cell Lysate (click product name for more information)

Product Name	Catalog #	Unit	Price	Qty	Add	Favorites
NIH/3T3 Whole Cell Lysate	sc-2210	500 µg/200 µl	$104

Ac-Histone H3 Background Information
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.

Ac-Histone H3 (Lys9/14) Product Citations

See how others have used Ac-Histone H3 (Lys9/14): sc-8655 antibody and or Ac-Histone H3 (Lys9/14) antibody conjugates.

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Ac-Histone H3 (Lys9/14)

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Ac-Histone H3 (Lys 9/14): sc-8655. Western blot analysis of acetylated Histone H3 expression in NIH/3T3 whole cell lysate.

Trichostatin A: sc-3511. Western blot analysis of Ac-Histone H3 acetylation in untreated (A,C) and Trichostatin A treated (B,D) NIH/3T3 whole cell lysates. Antibodies tested include: Ac-Histone H3 (Lys 9/14): sc-8655 (A,B) and Histone H3 (C-16): sc-8654 (C,D).

Ac-Histone H3 (Lys 9/14)-R: sc-8655-R. Western blot analysis of Ac-Histone H3 acetylation in untreated (A) and Trichostatin A (sc-3511) treated (B) NIH/3T3 whole cell lysates. Note upregulation of Ac-Histone H4 expression in lane B.

Western blot analysis of Ac-Histone H3 acetylation in untreated (A,C) and Trichostatin A (sc-3511) treated (B,D) NIH/3T3 whole cell lysates. Antibodies tested include Ac-Histone H3 (Lys 9/14)-R: sc-8655-R (A,B) and Histone H3 (C-16): sc-8654 (C,D).

Western blot analysis of Histone H3 acetylation in untreated (A) and Trapoxin A (sc-253730) treated (B) HeLa whole cell lysates. Antibody tested is Ac-Histone H3 (Lys 9/14)-R: sc-8655-R (A,B). Note acetylation of Ac-Histone H3 in lane B.

Western blot analysis of Histone H3 acetylation in untreated (A) and M 344 (sc-203124) treated (B) NIH/3T3 whole cell lysates. Antibody tested is Ac-Histone H3 (Lys 9/14)-R: sc-8655-R (A,B). Note acetylation of Histone H3 in lane B.

Western blot analysis of Histone H3 acetylation in untreated (A) and Apicidin (sc-202061) treated (B) HeLa whole cell lysates. Antibody tested is Ac-Histone H3 (Lys 9/14)-R: sc-8655-R (A,B). Note acetylation of Histone H3 in lane B.

Western blot analysis of Histone H3 acetylation in untreated (A) and Oxamflatin (sc-205960) treated (B) HeLa whole cell lysates. Antibody tested is Ac-Histone H3 (Lys 9/14)-R: sc-8655-R (A,B). Note acetylation of Histone H3 in lane B.

Ac-Histone H3 (Lys 9/14): sc-8655-R. Immunofluores-cence staining of methanol-fixed HeLa cells showing nuclear localization.

Western blot analysis of Histone H3 acetylation in untreated (A) and Sodium Butyrate (sc-202341) treated (B) HeLa whole cell lysates. Antibody tested is Ac-Histone H3 (Lys 9/14)-R: sc-8655-R (A,B). Note acetylation of Histone H3 in lane B.

Western blot analysis of Histone H3 acetylation in untreated (A) and Valproic Acid, Sodium Salt (sc-202378) treated (B) F9 whole cell lysates. Antibody tested is Ac-Histone H3 (Lys 9/14)-R: sc-8655-R (A,B). Note acetylation of Histone H3 in lane B.

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Ac-Histone H3 (Lys9/14) Antibody: sc-8655