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- rabbit polyclonal IgG, 200 µg/ml
- epitope mapping at the C-terminus of USF-2 of mouse origin
- recommended for detection of USF-2 p44 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine, bovine and porcine
- blocking peptide, sc-862 P
- TransCruz reagent for Gel Supershift and ChIP applications, sc-862 X, 200 µg/0.1 ml
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USF-2 Background Information
The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an upstream stimulatory factor that binds the core sequence CACGTG in the adenovirus late promoter. These findings, together with the demonstration of cooperative interaction between USF and the initiator-binding protein, TFII-I , raises the possibility of a more general involvement of USF in transcriptional regulation. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.
| USF-2 (C-20) Product Citations |
See how others have used USF-2 (C-20): sc-862 antibody and or USF-2 (C-20) antibody conjugates.
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USF-2 (C-20)
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USF-2 (C-20): sc-862. Western blot analysis of USF-2 p44 expression in NIH/3T3 (A) and KNRK (B) nuclear extracts.
USF-2 (C-20): sc-862. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear staining (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining (B).
USF-2 siRNA (h): sc-36786. Immunofluorescence staining of methanol-fixed, control HeLa (A) and USF-2 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with USF-2 (C-20): sc-862. USF-2 (C-20): sc-862. Western blot analysis of USF-2 expression in non-transfected: sc-117752 (A) and mouse USF-2 transfected: sc-127755 (B) 293T whole cell lysates and Jurkat nuclear extract (C). USF-2 (C-20): sc-862. Western blot analysis of USF-2 expression in non-transfected 293T: sc-117752 (A), mouse USF-2 transfected 293T: sc-127754 (B) and HeLa (C) whole cell lysates. |
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