USF-2 Background Information The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an upstream stimulatory factor that binds the core sequence CACGTG in the adenovirus late promoter. These findings, together with the demonstration of cooperative interaction between USF and the initiator-binding protein, TFII-I , raises the possibility of a more general involvement of USF in transcriptional regulation. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.
USF-2 (N-18) Product Citations
See how others have used USF-2 (N-18): sc-861 antibody and or USF-2 (N-18) antibody conjugates.
29 total citations
Loading citations.
USF-2 (N-18)
Click on image to enlarge
USF-2 (N-18): sc-861. Western blot analysis of USF-2 p44 expression in HeLa (A), Jurkat (B), K-562 (C), A-431 (D) and phorbol-induced NIH/3T3 (E) nuclear extracts.
USF-2 siRNA (h): sc-36786. Western blot analysis of USF-2 expression in non-transfected control (A) and USF-2 siRNA transfected (B) HeLa cells. Blot probed with USF-2 (N-18): sc-861. GAPDH (FL-335): sc-25778 used as specificity and loading control.
USF-2 (N-18): sc-861. Western blot analysis of USF-2 expression in non-transfected: sc-117752 (A) and mouse USF-2 transfected: sc-127755 (B) 293T whole cell lysates and Jurkat nuclear extract (C).
USF-2 (N-18): sc-861. Western blot analysis of USF-2 expression in non-transfected 293T: sc-117752 (A), mouse USF-2 transfected 293T: sc-127754 (B) and HeLa (C) whole cell lysates.
