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Cruz Tag 09™ (A-12) Antibody: sc-8600 |
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- goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-8600-R
- epitope mapping to the Cruz Tag 09 peptide domain of pCruz 09 mammalian expression vector
- agarose conjugate for IP studies, sc-8600 AC, 500 µg/0.25 ml agarose
- recommended for detection of fusion proteins encoded by the pCruz 09 mammalian expression vector by WB, IP, IF and ELISA
- blocking peptide, sc-8600 P
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CruzTag 09™ Background Information
Plasmid vectors are commonly used for the expression of hybrid fusion proteins, encoding a protein of interest fused to an epitope tag. Antibodies specific for such fusion protein tags are useful for monitoring expression of the protein of interest, both in cells and in Western blot analysis. Such epitope tags in common use include Myc, GST, His, HA and GFP. Santa Cruz Biotechnology introduces a novel series of epitope tags, called the CruzTags. The CruzTag epitope tags are encoded by the pCruz mammalian expression vectors, a group of unique and effective vectors suitable for the production of recombinant tagged fusion proteins. Each pCruz expression vector encodes a unique, 12 amino acid CruzTag epitope sequence. Anti-CruzTag antibodies are suitable for detection and immunoprecipitation of recombinant fusion proteins containing the respective CruzTag. |
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Cruz Tag 09™ (A-12)
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pCruz 09: sc-5040. Western blot analysis of Cruz Tag 09-Lac Z fusion protein expression in COS cells transfected with pCruz 09-Lac Z (A), pCruz 22-Lac Z (B) and non-transfected COS cells (C). Blot probed with Cruz Tag 09 (A-12)-R: sc-8600-R. |
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