epitope mapping at the C-terminus of Nibrin of human origin
recommended for detection of nibrin of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine and canine
Nibrin Background Information DNA repair proteins are necessary for the maintenance of chromosome integrity and are involved in the elimination of premutagenic lesions from DNA. The DNA repair proteins Rad51 and Rad52 are key components of the double-strand-break repair (DSBR) pathway. Rad51 is essential for mitotic and meiotic recombination, and its mutation in yeast and mammalian cells results in chromosome loss. Overexpression of Rad52 confers resistance to ionizing radiation and induces homologous intrachromosomal recombination. Rad52 is thought to be involved in an early stage of Rad51-mediated recombination. Additional proteins involved in the pathway include Dmc1 and nibrin. Dmc1 is specifically involved in meiotic recombination. Nibrin, which complexes with Mre11 and Rad50, is absent in Nijemegen breakage syndrome (NBS) patients.
Nibrin (C-19) Product Citations
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Nibrin (C-19)
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Nibrin (C-19): sc-8580. Western blot analysis of nibrin expression in HeLa nuclear extract.
Nibrin (C-19): sc-8580. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.
Nibrin siRNA (h): sc-36061. Western blot analysis of Nibrin expression in non-transfected control (A) and Nibrin siRNA transfected (B) HeLa cells. Blot probed with Nibrin (C-19): sc-8580. α-actinin (H-2): sc-17829 used as specificity and loading control.
Nibrin (C-19): sc-8580. Western blot analysis of Nibrin expression in HeLa whole cell lysate.
Nibrin (C-19): sc-8580. Western blot analysis of Nibrin expression in non-transfected: sc-117752 (A) and mouse Nibrin transfected: sc-125706 (B) 293T whole cell lysates.
Nibrin (C-19): sc-8580. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.
