epitope mapping near the N-terminus of Gfi-1 of human origin
recommended for detection of Gfi-1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine and bovine
blocking peptide, sc-8558 P
TransCruz reagent for Gel Supershift and ChIP applications, sc-8558 X, 200 µg/0.1 ml
Gfi-1 Background Information Growth factor independent 1 (Gfi-1) is a transcriptional repressor that specifically binds to the DNA consensus sequence TAAATCAC(A/T)GCA. The carboxy-terminus of Gfi-1 contains six C2H2-type zinc finger motifs, and zinc fingers 3, 4 and 5 are required for the binding of Gfi-1 to its DNA binding site. Gfi-1 also contains a 20 amino acid SNAG domain which mediates transcriptional repression. It represses Bax at the mRNA and protein levels, resulting in the inhibition of cell death. Gfi1 is expressed outside the lymphoid system in granulocytes and activated macrophages. Gfi-1B, a related protein, is a transcriptional repressor primarily expressed in bone marrow and spleen. Gfi-1B is a direct repressor of the p21 promoter and the Socs 1 and 3 promoters. The genes encoding human Gfi-1 and Gfi-1B map to chromosome 1p22 and 9q34.3, respectively.
Gfi-1 (N-20) Product Citations
See how others have used Gfi-1 (N-20): sc-8558 antibody and or Gfi-1 (N-20) antibody conjugates.
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Gfi-1 (N-20)
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Gfi-1 (N-20): sc-8558. Western blot analysis of Gfi-1 expression in THP-1 whole cell lysate.
Gfi-1 (N-20): sc-8558. Western blot analysis of Gfi-1 expression in non-transfected: sc-117752 (A) and human Gfi-1 transfected: sc-115318 (B) 293T whole cell lysates.
Gfi-1 (N-20): sc-8558. Western blot analysis of Gfi-1 expression in non-transfected: sc-117752 (A) and human Gfi-1 transfected: sc-159136 (B) 293T whole cell lysates.
Gfi-1 (N-20): sc-8558. Western blot analysis of Gfi-1 expression in human PBL whole cell lysate.
Gfi-1 (N-20): sc-8558. Western blot analysis of Gfi-1 expression in RBL-1 whole cell lysate.
Gfi-1 (N-20): sc-8558. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
