Actin Antibody (C-2): sc-8432

Actin Antibody (C-2) is a mouse monoclonal IgG1 kappa light chain antibody that specifically detects Actin, a highly conserved and essential cytoskeletal protein, across mouse, rat, human, and Xenopus laevis species. Anti-Actin antibody (C-2), with an epitope mapping between amino acids 357-375 at the C-terminus of Actin in human origin, is reactive in multiple assays such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Actin monoclonal antibody (C-2) is available in non-conjugated form and in multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® dyes. Actin is a ubiquitous protein expressed in all eukaryotic cells, constituting up to 50% of total cellular protein. Actin′s role in forming both stable and dynamic structures such as microvilli and the contractile apparatus of muscle cells underscores Actin′s importance in cellular architecture and motility. In mammals, at least six isoforms of Actin exist, divided into three main classes: α-Actin is found primarily in muscle cells, while β-Actin and γ-Actin are present in most other tissues. Actin plays a crucial role in cellular processes such as cell division, intracellular trafficking, and signal transduction. Actin organization within the cytoskeleton is tightly regulated by members of the small GTPase family, including Rho, which controls stress fiber assembly, Rac, which regulates plasma membrane-associated filaments, and Cdc42, which promotes filopodia formation. Actin′s critical role in maintaining cell structure and enabling dynamic cellular responses makes Actin a vital target in research for understanding fundamental biological processes and diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Actin Antibody (C-2) References:

1. Actin-based motility: from molecules to movement.  |  Carlier, MF., et al. 2003. Bioessays. 25: 336-45. PMID: 12655641
2. Vav1 controls DAP10-mediated natural cytotoxicity by regulating actin and microtubule dynamics.  |  Graham, DB., et al. 2006. J Immunol. 177: 2349-55. PMID: 16887996
3. [The role of smooth muscle alpha-actin in development of renal fibrosis in patients with chronic glomerulonephritis].  |  Chebotareva, NV., et al. 2006. Ter Arkh. 78: 17-21. PMID: 16889044
4. Assessing actin's growth rate.  |  Short, B. 2015. J Cell Biol. 208: 499. PMID: 25733710
5. Role of microtubule-associated proteins in the control of microtubule assembly.  |  Maccioni, RB. and Cambiazo, V. 1995. Physiol Rev. 75: 835-64. PMID: 7480164
6. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia.  |  Nobes, CD. and Hall, A. 1995. Cell. 81: 53-62. PMID: 7536630
7. A discourse on modeling F-actin.  |  Schutt, CE., et al. 1995. J Struct Biol. 115: 186-98. PMID: 7577239
8. Dynamic actin structures stabilized by profilin.  |  Finkel, T., et al. 1994. Proc Natl Acad Sci U S A. 91: 1510-4. PMID: 8108438
9. Actin cytoskeleton. Setting the pace of cell movement.  |  Barkalow, K. and Hartwig, JH. 1995. Curr Biol. 5: 1000-2. PMID: 8542272
10. The origins and evolution of eukaryotic proteins.  |  Doolittle, RF. 1995. Philos Trans R Soc Lond B Biol Sci. 349: 235-40. PMID: 8577833
11. Elastic fibres are an essential component of human placental stem villous stroma and an integrated part of the perivascular contractile sheath.  |  Graf, R., et al. 1996. Cell Tissue Res. 283: 133-41. PMID: 8581953
12. Actin bundles in human hair follicles as revealed by confocal laser microscopy.  |  Furumura, M. and Ishikawa, H. 1996. Cell Tissue Res. 283: 425-34. PMID: 8593672

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Actin Antibody (C-2) has 2,632 citations in a variety of scientific publications.

Primary antibodies like Anti-Actin Antibody (C-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Actin Antibody (C-2) Specifications

Alternate Product Name: β Actin

Product Clone Name: C-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 357-375

Epitope Target: Actin

Epitope Species: human

Product Reactivity/Detection: a broad range of Actin isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: Xenopus

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2,632 citations in various scientific publications

Available Conjugates/Forms for Actin Antibody (C-2): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, TRITC: Rhodamine

The preferred beta Actin antibody is beta Actin Antibody (C4): sc-47778.