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p-p70 S6 kinase α (A-6) Antibody: sc-8416 |
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- mouse monoclonal IgG1; 200 µg/ml
- raised against a sequence containing phosphorylated Ser 411 of p70 S6 kinase α isoform 2 of human origin
- recommended for detection of p-p70 S6 kinase α of mouse, rat and human origin phosphorylated at Ser 411 by WB, IP, IF, IHC(P) and ELISA
- blocking peptide, sc-8416 P
- agarose conjugate for IP studies, sc-8416 AC, 500 µg/0.25 ml agarose
- for phosphorylation positive (sc-2248) and non-activated control (sc-2257) cell extracts
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p-p70 S6 kinase α Background Information
In studies to elucidate key regulatory pathways in signal transduction, several protein serine/threonine (Ser/Thr) kinases have been identified. Included among such kinases are two distinct families of 40S ribosomal protein S6 Ser/Thr kinases present in somatic animal cells, designated p70 S6 kinase and p90 Rsk kinase. p90 Rsk kinase is maximally activated within minutes of addition of growth factors or phorbol ester to cultured cells followed by activation of p70 S6 kinase. Both enzymes are regulated by serine/threonine phosphorylation, suggesting that specific kinases may exist upstream in the signaling pathway that regulate these kinases. In fact, evidence suggests that one such family of activating enzymes includes the members of the ERK MAP kinase family. The ERK MAP kinases are, in turn, regulated by phosphorylation at threonine and tyrosine residues by a protein kinase designated MEK.
| p-p70 S6 kinase α (A-6) Product Citations |
See how others have used p-p70 S6 kinase α (A-6): sc-8416 antibody and or p-p70 S6 kinase α (A-6) antibody conjugates.
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p-p70 S6 kinase α (A-6)
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p-p70 S6 kinase α (A-6): sc-8416. Immunofluorescence staining of anisomycin-treated, methanol-fixed NIH/3T3 cells, showing cytoplasmic and nuclear localization of activated p-p70 S6 kinase α (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma showing nuclear localization of activated p-p70 S6 kinase α (B).
Western blot analysis of p-p70 S6 kinase α activation in whole cell lysates from serum-starved (A,C) and serum-starved then serum-treated (B,D) NIH/3T3 cells. Antibodies tested include p-p70 S6 kinase α (H-9): sc-8418 (A,B) and p-p70 S6 kinase α (A-6): sc-8416 (C,D). p-p70 S6 kinase α (A-6): sc-8416. Western blot analysis of p70 S6 kinase α phosphorylation in non-transfected 293T: sc-117752 (A), mouse p70 S6 kinase α transfected 293T: sc-125770 (B) and NIH/3T3 (C) whole cell lysates. p-p70 S6 kinase α (A-6): sc-8416. Western blot analysis of p70 S6 kinase α phosphorylation in untreated (A), insulin treated (B) and insulin treated and lambda protein phosphatase treated (C) 293T whole cell lysates.
Western blot analysis of p70 S6 kinase α phosphorylation in untreated (A,D), insulin treated (B,E) and insulin treated and lambda protein phosphatase ( sc-200312A) treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-p70 S6 kinase α (A-6): sc-8416 (A,B,C) and p70 S6 kinase α (C-18): sc-230 (D,E,F). Western blot analysis of p70 S6 kinase phosphorylation in untreated (A, D), insulin treated (B, E) and insulin and lambda protein phosphatase treated (C, F) 293T whole cell lysates. Antibodies tested include p-p70 S6 kinase (A-6): sc-8416 (A, B, C) and p70 S6 kinase (C-18): sc-230 (D, E, F). Western blot analysis of p70 S6 kinase phosphorylation in untreated (A,C) and lambda protein phosphatase treated (B,D) MCF7 whole cell lysates. Antibodies tested include p-p70 S6 kinase (A-6): sc-8416 (A,B) and p70 S6 kinase (C-18): sc-230 (C,D). |
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