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NFκB p50 (E-10) Antibody: sc-8414 |
| Datasheet |
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- mouse monoclonal IgG1; 200 µg/ml
- raised against amino acids 120-239 mapping at the N-terminus of NFκB p50 of human origin
- recommended for detection of NFκB p50 and NFκB p105 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA
- TransCruz reagent for Gel Supershift and ChIP applications, sc-8414 X, 200 µg/0.1 ml
- phycoerythrin conjugate for intracellular FCM, sc-8414 PE, 100 tests
- Alexa Fluor® 405 (sc-8418 AF405), Alexa Fluor® 488 (sc-8418 AF488) and Alexa Fluor® 647 (sc-8418 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
NFKB1 |
4790 |
4q24 |
NM_003998 |
P19838
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164011 |
| Mouse |
Nfkb1 |
18033 |
3 G3 |
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P25799
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N/A |
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NFκB p50 Background Information
Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NF˚B (p50 and p65) and the Drosophila maternal morphagen, dorsal. These proteins share sequence homology over a region of 300 amino acids at their NH2-terminus, the region that contains their DNA binding and dimerization domains. The DNA binding activity of NF˚B is activated and rapidly transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins have been described. These proteins, designated p105 and p100, are highly related but map on different chromosomes. The p105 (p110) precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated PdI, binds to p50 and regulates its activity.
| NFκB p50 (E-10) Product Citations |
See how others have used NFκB p50 (E-10): sc-8414 antibody and or NFκB p50 (E-10) antibody conjugates.
63 total citations Loading citations.
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| Alexa Fluor® is a
trademark of Molecular Probes Inc., OR., USA |
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NFκB p50 (E-10)
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NFκB p50 (E-10): sc-8414. Immunofluroescence staining of methanol-fixed K-562 cells showing nuclear staining.
NFκB p50 (E-10): sc-8414. Western blot analysis of NFκB p50 and p105 expression in K-562 whole cell lysate.
NFκB p50 (E-10) PE: sc-8414 PE. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-2866. ChIP analysis of cofactor occupancy dynamics on the KAI1 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, HDAC3 (H-99): sc-11417, HDAC3 (N-19): sc-8138, Bcl-3 (C-14): sc-185, Bcl-3 (H-146): sc-13038. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
ChIP analysis of cofactor occupancy dynamics on the ICAM1 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, Bcl-3 (C-14): sc-185, Bcl-3 (H-146): sc-13038, PCAF (C-16): sc-6300, PCAF (H-369): sc-8999, CBP (A-22): sc-369, CBP (C-1): sc-7300, CBP (C-20): sc-583, CBP (451): sc-1211. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
ChIP analysis of cofactor occupancy dynamics on the IL-8 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, CBP (A-22): sc-369, CBP (C-1): sc-7300, CBP (C-20): sc-583, CBP (451): sc-1211, p300 (C-20): sc- sc-585, p300 (N-15): sc-584, p300 (H-272): sc-8981. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67. NFκB p50 (E-10) Alexa Fluor 488: sc-8414 AF488. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-3890. NFκB p50 (E-10) Alexa Fluor 647: sc-8414 AF647. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-24636. NFκB p50 (E-10): sc-8414. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization using indirect FITC (A) staining and HeLa cells showing cytoplasmic localization using direct Alexa Fluor 488 (B) staining.
Western blot analysis of NFκB p50 phosphorylation in untreated (A,D), TNFα and calyculin A treated (B,E) and TNFα, calyculin A and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-NFκB p50 (A-8): sc-271908 (A,B,C) and NFκB p50 (E-10): sc-8414 (D,E,F). NFκB p50 (E-10): sc-8414. Western blot analysis of NFκB p50 expression in non-transfected: sc-117752 (A) and human NFκB p50 transfected: sc-116112 (B) 293T whole cell lysates. NFκB p50 (E-10): sc-8414. Western blot analysis of NFκB p50 expression in non-transfected: sc-117752 (A) and mouse NFκB p50 transfected: sc-122025 (B) 293T whole cell lysates. Western blot analysis of NFκB p50 phosphorylation in untreated (A,D), TNF and calyculin A treated (B,E) and TNF, calyculin A and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-NFκB p50 (Ser 932): sc-101747 (A,B,C) and NFκB p50 (E-10): sc-8414 (D,E,F). Western blot analysis of NFκB p50 phosphorylation in untreated (A,D), calyculin A treated (B,E) and calyculin A and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-NFκB p50 (Ser 932): sc-101747 (A,B,C) and NFκB p50 (E-10): sc-8414 (D,E,F). |
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