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home » Kinases and Phosphatases » MEK Antibody / MEK Antibodies » p-MEK-3/6 (B-9): sc-8407

p-MEK-3/6 (B-9) Antibody: sc-8407

  |  Datasheet
  • mouse monoclonal IgG1; 200 µg/ml
  • recommended for detection of MEK-3/6 of mouse, rat and human origin phosphorylated at Ser 189 and Ser 207 by WB, IP, IF, IHC(P) and ELISA
  • for phosphorylation positive (sc-2258) and non-activated control (sc-2200) cell extracts
  • blocking peptide, sc-8407 P
 
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Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human MAP2K3 5606 17p11.2 NM_002756, NM_145109, NM_145110 P46734
 602315
Mouse Map2k3 26397 11 B2 O09110
 N/A
Mouse Map2k6 26399 11 E2 P70236
 N/A
 
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p-MEK-3/6 (B-9) sc-8407 200 µg/ml $279
p-MEK-3/6 (B-9) P sc-8407 P
(peptide)
 100 µg/0.5 ml $61
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
MEK-3/6 siRNA (h) sc-43924 10 µM $258
MEK-3/6 (h)-PR sc-43924-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
MEK-3/6 shRNA Plasmid (h) sc-43924-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
MEK-3/6 shRNA (h) Lentiviral Particles sc-43924-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
MEK-3 (h): 293T Lysate sc-114954 100µg/200µl $205
MEK-6 (h): 293T Lysate sc-113820 100µg/200µl $205
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
Sol8 Cell Lysate sc-2249 500 µg/200 µl $104
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
HeLa + PMA Cell Lysate sc-2258 500 µg/200 µl $104
KNRK Whole Cell Lysate sc-2214 500 µg/200 µl $104

MEK-3/6 Background Information
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK (1). A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity (2). MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases (3). MEK-5 appears to specifically phosphorylate ERK5 (4), whereas MEK-6 phosphorylates p38 and p38b (5,6). MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway (7-9).

p-MEK-3/6 (B-9) Product Citations
See how others have used p-MEK-3/6 (B-9): sc-8407 antibody and or p-MEK-3/6 (B-9) antibody conjugates.


12 total citations
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p-MEK-3/6 (B-9)
Click on image to enlarge
sc-8407
Western blot analysis of MEK-3/MEK-6 activation in untreated (A,C) and PMA-induced (B,D) HeLa cells. Antibodies tested include a phospho-specific monoclonal, p-MEK-3/6 (B-9): sc-8407 (A,B) and a control antibody, MEK-3 (C-19): sc-961 (C,D).
sc-8407
p-MEK-3/6 (B-9): sc-8407. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic staining.
sc-8407
p-MEK-3/6 (B-9): sc-8407. Western blot analysis of MEK-3 phosphorylation in non-transfected 293T: sc-117752 (A), human MEK-3 transfected 293T: sc-114954 (B) and Jurkat (C) whole cell lysates.
sc-8407
p-MEK-3/6 (B-9): sc-8407. Immunofluorescence staining of methanol-fixed, phosphorylated HeLa cells showing nuclear and cytoplasmic localization.
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