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- mouse monoclonal IgG2b; 200 µg/ml
- recommended for detection of IκB-α of mouse, rat and human origin phosphorylated at Ser 32 by WB, IP, IF, IHC(P) and ELISA
- blocking peptide, sc-8404 P
- HRP conjugate, sc-8404 HRP, 200 µg/ml
- for phosphorylation positive (sc-2228) and non-activated control (sc-2200) cell extracts
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
NFKBIA |
4792 |
14q13.2 |
NM_020529 |
P25963
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164008 |
| Mouse |
Nfkbia |
18035 |
12 C1 |
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Q9Z1E3
|
N/A |
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IκB-α Background Information
On the basis of both functional and structural considerations, members of the I˚B family of proteins can be divided into four groups. The first of these groups, I˚B-å, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to ˚B sites, suggesting that the I˚B-å family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the I˚B family is represented by a protein designated I˚B-∫. The third group of I˚B proteins is represented by I˚B-©, a protein identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and expressed predominantly in lymphoid cells. An additional I˚B family member has been identified as I˚B-e, a protein which has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel.
| p-IκB-α (B-9) Product Citations |
See how others have used p-IκB-α (B-9): sc-8404 antibody and or p-IκB-α (B-9) antibody conjugates.
57 total citations Loading citations.
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p-IκB-α (B-9)
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Western blot analysis of IκB-α activation in untreated (A,C) and TNF&alpha-induced (B,D) HeLa cells. Antibodies tested include a phospho-specific IκB-α monoclonal, p-IκB-α (B-9): sc-8404 (A,B) and a control antibody, IκB-α (H-4): sc-1643 (C,D). p-IκB-α (B-9): sc-8404. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma showing nuclear localization of activated IκB-α (A). Immunofluorescence staining of methanol-fixed, TNFα-treated HeLa cells, showing nuclear localization of activated IκB-α (B).
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