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home » Transcription Regulators » ATF Antibody / ATF Antibodies » p-ATF-2 (F-1): sc-8398

p-ATF-2 (F-1) Antibody: sc-8398

  |  Datasheet
  • mouse monoclonal IgG1; 200 µg/ml
  • epitope corresponding to a short amino acid sequence containing Thr 71 phosphorylated ATF-2 of human origin
  • recommended for detection of ATF-2 phosphorylated at Thr 71 and correspondingly phosphorylated ATF-7 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including equine, bovine, porcine and avian
  • blocking peptide, sc-8398 P
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-8398 X, 200 µg/0.1 ml
  • agarose conjugate for IP studies, sc-8398 AC, 500 µg/0.25 ml agarose
  • fluorescein (sc-8398 FITC) and rhodamine (sc-8398 TRITC) conjugates for immunofluorescence, 200 µg/ml
  • for phosphorylation positive (sc-2247) and non-activated control (sc-2210) cell extracts
  • Alexa Fluor® 405 (sc-8398 AF405), Alexa Fluor® 488 (sc-8398 AF488) and Alexa Fluor® 647 (sc-8398 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
 
Additional ATF Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   FCM   Gel Shift   ChIP  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human ATF2 1386 2q31.1 NM_001880 P15336
 123811
Mouse Atf2 11909 2 C3 P16951
 N/A
 
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p-ATF-2 (F-1) sc-8398 200 µg/ml $279
p-ATF-2 (F-1) AC sc-8398 AC 500µg/ml, 25%ag $366
p-ATF-2 (F-1) Alexa Fluor® 405 sc-8398 AF405 100 µg/2 ml $314
p-ATF-2 (F-1) Alexa Fluor® 488 sc-8398 AF488 100 µg/2 ml $314
p-ATF-2 (F-1) Alexa Fluor® 647 sc-8398 AF647 100 µg/2 ml $314
p-ATF-2 (F-1) FITC sc-8398 FITC 100 tests in 2 ml $292
p-ATF-2 (F-1) P sc-8398 P
(peptide)
 100 µg/0.5 ml $61
p-ATF-2 (F-1) TRITC sc-8398 TRITC 200 µg/ml $282
p-ATF-2 (F-1) X sc-8398 X 200 µg/0.1 ml $279
 WB Positive Control Cell Lysate (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NIH/3T3 + anisomycin Cell Lysate sc-2247 500 µg/200 µl $104

ATF-2 Background Information
Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promotor and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro. The gene encoding human ATF-2 maps to chromosome 2q31.1.

p-ATF-2 (F-1) Product Citations
See how others have used p-ATF-2 (F-1): sc-8398 antibody and or p-ATF-2 (F-1) antibody conjugates.


16 total citations
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Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
 
p-ATF-2 (F-1)
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sc-8398
Western blot analysis of ATF-2 activation in untreated (A,C) and anisomycin-induced (B,D) NIH/3T3 cells. Antibodies tested include a phospho-specific ATF-2 monoclonal, p-ATF-2 (F-1): sc-8398 (A,B) and a control antibody, ATF-2 (N-96): sc-6233 (C,D).
sc-8398
p-ATF-2 (F-1): sc-8398. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization of activated ATF-2.
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