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p-Stat1 Antibody (A-2): sc-8394

 |  Datasheet

(Based on data analysis)

  • p-Stat1 Antibody (A-2) is a mouse monoclonal IgG1 provided at 200 µg/ml
  • recommended for detection of Stat1α p91 and Stat1β p84 of mouse, rat and human origin phosphorylated at Tyr 701 by WB, IP, IF, FCM and ELISA
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-8394 X, 200 µg/0.1 ml
  • fluorescein (sc-8394 FITC), phycoerythrin (sc-8394 PE), PerCP (sc-8394 PerCP) and PerCP-Cy5.5 (sc-8394 PCPC5) conjugate for intracellular FCM, 100 tests
  • for phosphorylation positive (sc-2222) and non-activated control (sc-2200) cell extract
  • blocking peptide, sc-8394 P
  • See 26 citations for p-Stat1 Antibody (A-2)
 

See additional Stat Antibodies including Stat, Stat1, Stat5A/B, Stat2, Stat3, Stat4, Stat5 and Stat6.

Also see Stat Inhibitors for functional analysis of cellular responses to Stat.


Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   FCM   Gel Shift   ChIP   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-Stat1 Antibody (A-2) sc-8394 200 µg/ml $279
p-Stat1 Antibody (A-2) FITC sc-8394 FITC 100 tests in 2 ml $292
p-Stat1 (A-2) P sc-8394 P
(peptide)
 100 µg/0.5 ml $61
p-Stat1 Antibody (A-2) PCPC5 sc-8394 PCPC5 100 tests in 2 ml $310
p-Stat1 Antibody (A-2) PE sc-8394 PE 100 tests in 2 ml $303
p-Stat1 Antibody (A-2) PerCP sc-8394 PerCP 100 tests in 2 ml $310
p-Stat1 Antibody (A-2) X sc-8394 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Stat1 p84/p91 siRNA (h) sc-44123 10 µM $258
Stat1 p84/p91 siRNA (m) sc-44124 10 µM $258
Stat1 p84/p91 (h)-PR sc-44123-PR 10 µM, 20 µl $23
Stat1 p84/p91 (m)-PR sc-44124-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Stat1 p84/p91 shRNA Plasmid (h) sc-44123-SH 20 µg $520
Stat1 p84/p91 shRNA Plasmid (m) sc-44124-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Stat1 p84/p91 shRNA (h) Lentiviral Particles sc-44123-V 200 µl $625
Stat1 p84/p91 shRNA (m) Lentiviral Particles sc-44124-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HeLa + IFN-γ Cell Lysate sc-2222 500 µg/200 µl $104
SK-MEL-28 + IFN-γ Cell Lysate sc-2291 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human STAT1 6772 2q32.2 NM_007315, NM_139266 P42224
 600555
Mouse Stat1 20846 1 C1.1 P42225
 N/A
 

Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors (1,2). Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex (3). Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3∫ appears to be activated by both while Stat3å is activated by EGF, but not by IL-6 (4,5). Highest expression of Stat4 is seen in testis and myeloid cells (6). IL-12 has been identified as an activator of Stat4 (2). Stat5 has been shown to be activated by prolactin and by IL-3 (7). Stat6 is involved in IL-4 activated signaling pathways (8).

p-Stat1 Antibody (A-2) Product Citations
See how others have used p-Stat1 Antibody (A-2) and or p-Stat1 Antibody (A-2) conjugates.


26 total citations
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p-Stat1 Antibody (A-2) Data
Click on image to enlarge
sc-8394
Western blot analysis of Stat1 activation in untreated (A,C) and γ-interferon-induced (B,D) HeLa cells. Antibodies tested include a phospho-specific Stat1 monoclonal, p-Stat1 (A-2): sc-8394 (A,B) and a control antibody, Stat1α p91 (C-111): sc-417 (C,D).
sc-8394
p-Stat1 (A-2) PE: sc-8394 PE. Intracellular FCM analysis of fixed and permeabilized control (green line histogram) and IFN-γ-induced (solid orange histogram) U-937 cells. Dotted pink histogram represents the isotype control, normal mouse IgG1: sc-2866.
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