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- rabbit polyclonal IgG, 200 µg/ml
- epitope corresponding to amino acids 1-238 representing full length GFP (green fluorescent protein) of Aequorea victoria origin
- recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF and ELISA
- agarose conjugate for IP studies, sc-8334 AC, 500 µg/0.25 ml agarose
- HRP conjugate, sc-8334 HRP, 200 µg/ml
- biotin conjugate, sc-8334 B, 200 µg/ml
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GFP Background Information
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
| GFP (FL) Product Citations |
See how others have used GFP (FL): sc-8334 antibody and or GFP (FL) antibody conjugates.
184 total citations Loading citations.
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GFP (FL)
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GFP (FL): sc-8334. Fluorescence staining of methanol-fixed COS cells transfected with pCruz GFP-Lac Z: sc-5046, showing GFP-Lac Z localization in the cytoplasm. GFP (FL): sc-8334. Western blot analysis of GFP fusion protein expression in non-transfected COS cells (A) and COS cells transfected with pCruz GFP-Lac Z: sc-5046 (B). |
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