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PRAK Antibody (N-19): sc-8251

 |  Datasheet

(Based on data analysis)

  • PRAK Antibody (N-19) is a goat polyclonal IgG provided at 200 µg/ml
  • epitope mapping at the N-terminus of PRAK of human origin
  • recommended for detection of PRAK of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine and porcine
  • blocking peptide, sc-8251 P
 

See additional PRAK Antibodies.

Also see PRAK Inhibitors for functional analysis of cellular responses to PRAK.


Ordering InformationGene Info
Recommended Support Products:
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WB   IP   IF   siRNA  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
PRAK Antibody (N-19) sc-8251 200 µg/ml $279
PRAK (N-19) P sc-8251 P
(peptide)
 100 µg/0.5 ml $61
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
PRAK siRNA (h) sc-36310 10 µM $258
PRAK siRNA (m) sc-36311 10 µM $258
PRAK (h)-PR sc-36310-PR 10 µM, 20 µl $23
PRAK (m)-PR sc-36311-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
PRAK shRNA Plasmid (h) sc-36310-SH 20 µg $520
PRAK shRNA Plasmid (m) sc-36311-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
PRAK shRNA (h) Lentiviral Particles sc-36310-V 200 µl $625
PRAK shRNA (m) Lentiviral Particles sc-36311-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
PRAK (h): 293T Lysate sc-116012 100 µg/200 µl $205
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human MAPKAPK5 8550 12q24.12 NM_003668, NM_139078 Q8IW41
 606723
Mouse Mapkapk5 17165 5 F O54992
 N/A
 

PRAK (p38-regulated/activated kinase), also referred to as mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK)-5, is a ubiquitously expressed serine/threonine kinase regulated by p38å and p38ß MAP kinases. Activated JNK, p38g or p38∂ are unable to induce phosphorylation of PRAK in vitro. Phosphorylation of PRAK occurs in vivo in response to p38 activation by stress-related extracellular stimuli including UV light, oxidation and proinflammatory cytokines. Two other substrates for p38, MAPKAPK-2 and MAPKAPK-3/3pK, share approximately 45% sequence homology with PRAK including the phosphorylation motif recognized by p38, Lys-X-Thr-Pro. Activated PRAK has been shown to specifically phosphorylate HSP 27 in vitro, suggesting that the protein may play a role in stress-induced small heat shock protein phosphorylation in vivo.
PRAK Antibody (N-19) Data
Click on image to enlarge
sc-8251
PRAK (N-19): sc-8251. Western blot analysis of PRAK expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.
sc-8251
PRAK (N-19): sc-8251. Western blot analysis of PRAK expression in non-transfected: sc-117752 (A) and human PRAK transfected: sc-116012 (B) 293T whole cell lysates.
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