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p-c-Jun Antibody (KM-1): sc-822

 |  Datasheet

(Based on data analysis)

  • p-c-Jun Antibody (KM-1) is a mouse monoclonal IgG1 provided at 200 µg/ml
  • epitope corresponding to amino acids 56-69 of human c-Jun
  • recommended for detection of c-Jun p39 phosphorylated on Serine 63 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM; non cross-reactive with Jun B or Jun D phosphorylated on the analogous serine residues or with c-Jun non-phosphorylated at Serine 63; also reactive with additional species, including canine, bovine, porcine and avian
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-822 X, 200 µg/0.1 ml
  • HRP conjugate, sc-822 HRP, 200 µg/ml
  • fluorescein (sc-822 FITC), phycoerythrin (sc-822 PE), PerCP (sc-822 PerCP) and PerCP-Cy5.5 (sc-822 PCPC5) conjugates for intracellular FCM, 100 tests
  • for phosphorylation positive (sc-2247) and non-activated control (sc-2210) cell extracts
  • Alexa Fluor® 405 (sc-822 AF405), Alexa Fluor® 488 (sc-822 AF488) and Alexa Fluor® 647 (sc-822 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
  • See 186 citations for p-c-Jun Antibody (KM-1)
 

See additional Jun Antibodies including Jun, c-Jun, Jun B and Jun D.

Also see c-Jun Activators for functional analysis of cellular responses to c-Jun.


Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   FCM   Gel Shift   ChIP   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-c-Jun Antibody (KM-1) sc-822 200 µg/ml $279
p-c-Jun Antibody (KM-1) Alexa Fluor® 405 sc-822 AF405 100 µg/2 ml $314
p-c-Jun Antibody (KM-1) Alexa Fluor® 488 sc-822 AF488 100 µg/2 ml $314
p-c-Jun Antibody (KM-1) Alexa Fluor® 647 sc-822 AF647 100 µg/2 ml $314
p-c-Jun Antibody (KM-1) FITC sc-822 FITC 100 tests in 2 ml $292
p-c-Jun Antibody (KM-1) HRP sc-822 HRP 200 µg/ml $279
p-c-Jun Antibody (KM-1) PCPC5 sc-822 PCPC5 100 tests in 2 ml $310
p-c-Jun Antibody (KM-1) PE sc-822 PE 100 tests in 2 ml $303
p-c-Jun Antibody (KM-1) PerCP sc-822 PerCP 100 tests in 2 ml $310
p-c-Jun Antibody (KM-1) X sc-822 X 200 µg/0.1 ml $279
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
c-Jun siRNA (h) sc-29223 10 µM $258
c-Jun siRNA (m) sc-29224 10 µM $258
c-Jun (h)-PR sc-29223-PR 10 µM, 20 µl $23
c-Jun (m)-PR sc-29224-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
c-Jun shRNA Plasmid (h) sc-29223-SH 20 µg $520
c-Jun shRNA Plasmid (m) sc-29224-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
c-Jun shRNA (h) Lentiviral Particles sc-29223-V 200 µl $625
c-Jun shRNA (m) Lentiviral Particles sc-29224-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
A-431 nuclear extract sc-2122 250 µg/0.05 ml $143
HeLa + UV Cell Lysate sc-2221 500 µg/200 µl $104
c-Jun (m2): 293T Lysate sc-125070 100 µg/200 µl $205
c-Jun (h): CHO Lysate sc-110019 100 µg/200 µl $205
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
NIH/3T3 + anisomycin Cell Lysate sc-2247 500 µg/200 µl $104
c-Jun (m): 293T Lysate sc-125069 100 µg/200 µl $205
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human JUN 3725 1p32.1 NM_002228 P05412
 165160
Mouse Jun 16476 4 C5 P05627
 N/A
 

Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are found to be associated with a number of transcriptional complexes (1,2). The c-Jun protein is a major component of the transcription factor AP-1 (3,4), originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE) (3,4). The Jun proteins form homo- and heterodimers which bind the TRE, but the Fos proteins are active only as heterodimers with any of the Jun proteins (5,6). Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain (7). An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is deactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA) (8).

p-c-Jun Antibody (KM-1) Product Citations
See how others have used p-c-Jun Antibody (KM-1) and or p-c-Jun Antibody (KM-1) conjugates.


186 total citations
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p-c-Jun Antibody (KM-1) Data
Click on image to enlarge
sc-822
c-Jun (KM-1): sc-822. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing punctate nuclear staining of phosphorylated c-Jun (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma showing nuclear localization of activated c-Jun (B).
sc-822
Western blot analysis of c-Jun activation in whole cell lysates from control (A) and anisomycin-treated (B) NIH/3T3 cells and phorbol ester-induced A-431 nuclear extracts (C,D,E). Antibodies tested include p-c-Jun (KM-1): sc-822 (A,B,C) and p-c-Jun (KM-1): sc-822 blocked with its cognate (D) or an unrelated peptide (E).
sc-822
p-c-Jun (KM-1) Alexa Fluor 488: sc-822 AF488. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-3890.
sc-822
p-c-Jun (KM-1): sc-822. Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125069 (B) 293T whole cell lysates.
sc-822
p-c-Jun (KM-1): sc-822. Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125070 (B) 293T whole cell lysates.
sc-822
p-c-Jun (KM-1): sc-822. Western blot analysis of c-Jun phosphorylation in non-transfected CHO: sc-117750 (A), human c-Jun transfected CHO: sc-110019 (B) and NIH/3T3 (C) whole cell lysates.
sc-822
Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A, D), untreated mouse c-Jun transfected: sc-125069 (B, E) and lambda protein phosphatase treated mouse c-Jun transfected: sc-125069 (C, F) 293T whole cell lysates. Antibodies tested include p-c-Jun (KM-1): sc-822 (A, B, C) and c-Jun (N): sc-45 (D, E, F).
sc-822
p-c-Jun (KM-1): sc-822. Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A) and human c-Jun transfected: sc-159456 (B) 293T whole cell lysates.
sc-822
Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse c-Jun transfected: sc-125069 (B,E) and lambda protein phosphatase (sc-200312A) treated human c-Jun transfected: sc-125069 (C,F) 293T whole cell lysates. Antibodies tested include p-c-Jun (KM-1): sc-822 (A,B,C) and c-Jun (H-79): sc-1694 (D,E,F).
sc-822
Western blot analysis of c-Jun phosphorylation in untreated (A,D), UV irradiated (B,E) and UV irradiated and lambda protein phosphatase (sc-200312A) treated (C,F) NIH/3T3 whole cell lysates. Antibodies tested include p-c-Jun (KM-1): sc-822 (A,B,C) and c-Jun (H-79): sc-1694 (D,E,F).
sc-822
Western blot analysis of human recombinant c-Jun (A,C) and human recombinant c-Jun phosphorylated by human recombinant JNK1 (B,D) fusion proteins. Antibodies tested include c-Jun (N): sc-45 (A,B) and p-c-Jun (KM-1): sc-822 (C,D).
sc-822
Anisomycin: sc-3524. Intracellular FCM analysis of Anisomycin (sc-3524) induced c-Jun serine-63 phosphorylation: Pink dotted lines represent mouse IgG1 PE stained Anisomycin (sc-3524) treated NIH/3T3 cells. Green line histograms represent untreated NIH/3T3 cells. Solid orange histograms represent anisomycin treated NIH/3T3 cells. p-c-Jun (KM-1) PE: sc-822 PE.
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