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home » Cell Cycle Proteins » p21 Antibody / p21 Antibodies » p21 (187): sc-817

p21 (187) Antibody: sc-817

  |  Datasheet
  • mouse monoclonal IgG1; 200 µg/ml
  • produced by immunization with full length p21 of human origin
  • recommended for detection of p21 of human origin by WB, IP, IF, IHC(P) and ELISA
  • TransCruz reagent for ChIP application, sc-817 X, 200 µg/0.1 ml
 
Additional p21 Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   ChIP   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human CDKN1A 1026 6p21.31 NM_000389, NM_078467 P38936
 116899
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p21 (187) sc-817 200 µg/ml $279
p21 (187) X sc-817 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
p21 siRNA (h) sc-29427 10 µM $258
p21 siRNA (h2) sc-44214 10 µM $258
p21 (h)-PR sc-29427-PR 10 µM $23
p21 (h2)-PR sc-44214-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
p21 shRNA Plasmid (h) sc-29427-SH 20 µg $520
p21 shRNA Plasmid (h2) sc-44214-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
p21 shRNA (h) Lentiviral Particles sc-29427-V 200 µl $625
p21 shRNA (h2) Lentiviral Particles sc-44214-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
C32 nuclear extract sc-2136 1000 µg $143
C32 + PMA nuclear extract sc-2137 1000 µg $143
C32 Whole Cell Lysate sc-2205 500 µg/200 µl $104
HeLa nuclear extract sc-2120 1000 µg $143
MDA-MB-231 Cell Lysate sc-2232 500 µg/200 µl $104
MCF7 Whole Cell Lysate sc-2206 500 µg/200 µl $104

p21 Background Information
It is now well established that cyclins play a positive role in promoting cell cycle transitions via their ability to associate with and activate their cognate cyclin-dependent kinases (Cdks). Cdk2 associates with cyclins A, D and E, and has been implicated in the control of the G1 to S phase transition in mammals. A novel Cdk-interacting protein, designated p21, Cip1 or WAF1, has been identified in cyclin A, cyclin D1, cyclin E and Cdk2 immunoprecipitates. p21 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk 2, cyclin E-Cdk2, cyclin D1-Cdk4 and cyclin D2-Cdk4 complexes. Expression of p21 is inducible by wildtype, but not mutant, p53. The mouse homolog of p21 is designated CAP20.

p21 (187) Product Citations
See how others have used p21 (187): sc-817 antibody and or p21 (187) antibody conjugates.


88 total citations
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p21 (187)
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sc-817
p21 (187): sc-817. Western blot analysis of human (h) and mouse (m) p21 expressed in E. coli. Tested at 1:20,000 against both human (h) and mouse (m) p21 at 100, 20, 10 and 5 ng per lane.
sc-817
p21 (187): sc-817. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing nuclear staining of ductal epithelia (A). Immunofluorescence staining of a mixed population of NIH/3T3 cells transfected with human p21 cDNA. Note nuclear staining of a p21 positive cell (B).
sc-817
Western blot analysis of p21 expression in untreated (A,C,E) and phorbol ester-induced (B,D,F) C32 nuclear extracts. Antibodies tested include p21 (187): sc-817 (A,B), p21 (C-19)-G: sc-397-G (C,D) and p21 (C-19): sc-397 (E,F).
sc-817
p21 (187): sc-817. Western blot analysis of p21 expression in C32 whole cell lysate.
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