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p-ERK 1/2 Antibody (12D4): sc-81492

 |  Datasheet

(Based on data analysis)

  • p-ERK 1/2 Antibody (12D4) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
  • raised against synthetic phosphopeptide corresponding to amino acid residues surrounding the T-E-Y motif of ERK1 of human origin
  • recommended for detection of Thr 202 and Tyr 204 dually phosphorylated ERK 1 and ERK 2 of mouse, rat and human origin by WB, IP and IF; non cross-reactive with non-phosphorylated ERK 1/2. Requires phosphorylation at both the Thr and Tyr site; also reactive with additional species, including canine
  • See 14 citations for p-ERK 1/2 Antibody (12D4)
 

See additional ERK Antibodies including ERK, ERK 1, ERK 2, ERK 3, ERK 4 and ERK 5.

Also see ERK 1 Inhibitors, ERK 1 Activators or ERK 1 Substrates for functional analysis of cellular responses to ERK 1.


Ordering InformationProduct CitationsGene Info
Recommended Support Products:
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WB   IP   IF  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-ERK 1/2 Antibody (12D4) sc-81492 50 µg/0.5 ml $279
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
HeLa + TNFα Cell Lysate sc-2228 500 µg/200 µl $104
Jurkat + PMA Cell Lysate sc-24718 500 µg/200 µl $104
NIH/3T3 + PDGF Cell Lysate sc-3803 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
HeLa + UV Cell Lysate sc-2221 500 µg/200 µl $104
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human MAPK1 5594 22q11.21 NM_002745, NM_138957 P28482
 176948
Human MAPK3 5595 16p11.2 NM_001040056, NM_002746 P27361
 601795
Mouse Mapk1 26413 16 A3 P63085
 N/A
Mouse Mapk3 26417 7 F3 Q63844
 N/A
 

The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively (1–3). ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif (4,5). Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation (6). The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop (6). In response to activation, MAP kinases phosphorylate downstream components on serine and threonine (4,5). Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1 (5). The ERK family has three additional members: ERK 3, ERK 5 and ERK 6 (7,8).

p-ERK 1/2 Antibody (12D4) Product Citations
See how others have used p-ERK 1/2 Antibody (12D4) and or p-ERK 1/2 Antibody (12D4) conjugates.


14 total citations
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p-ERK 1/2 Antibody (12D4) Data
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sc-81492
p-ERK 1/2 (12D4): sc-81492. Western blot analysis of ERK 1/2 phosphorylation in non-stimulated (A), EGF stimulated (B) and pervanadate treated (C) SKOV3 whole cell lysates.
sc-81492
Western blot analysis of ERK 1/2 phosphorylation in untreated (A, D), PMA treated (B, E) and PMA and lambda protein phosphatase treated (C, F) Jurkat whole cell lysates. Antibodies tested include p-ERK 1/2 (12D4): sc-81492 (A, B, C) and ERK 2 (K-23): sc-153 (D, E, F).
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