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Jun B (C-11) Antibody: sc-8051

 |  Datasheet
  • mouse monoclonal IgG1; 200 µg/ml
  • specific for an epitope mapping between amino acids 200-222 near the C-terminus of Jun B of mouse origin
  • recommended for detection of Jun B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-8051 X, 200 µg/0.1 ml
  • agarose conjugate for IP studies, sc-8051 AC, 500 µg/0.25 ml agarose
  • fluorescein (sc-8051 FITC) and rhodamine (sc-8051 TRITC) conjugates for immunofluorescence, 200 µg/ml
  • blocking peptide, sc-8051 P
  • Alexa Fluor® 405 (sc-8051 AF405), Alexa Fluor® 488 (sc-8051 AF488) and Alexa Fluor® 647 (sc-8051 AF647) conjugates for immunofluorescence; 100 µg/2 ml
 
Additional c-Jun Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   Gel Shift   ChIP   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human JUNB 3726 19p13.13 NM_002229 P17275
165161
Mouse Junb 16477 8 C3 P09450
N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
Jun B (C-11) sc-8051 200 µg/ml $279
Jun B (C-11) AC sc-8051 AC 500µg/ml, 25%ag $366
Jun B (C-11) Alexa Fluor® 405 sc-8051 AF405 100 µg/2 ml $314
Jun B (C-11) Alexa Fluor® 488 sc-8051 AF488 100 µg/2 ml $314
Jun B (C-11) Alexa Fluor® 647 sc-8051 AF647 100 µg/2 ml $314
Jun B (C-11) FITC sc-8051 FITC 200 µg/ml $282
Jun B (C-11) P sc-8051 P
(peptide)
100 µg/0.5 ml $61
Jun B (C-11) TRITC sc-8051 TRITC 200 µg/ml $282
Jun B (C-11) X sc-8051 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Jun B siRNA (h) sc-35726 10 µM $258
Jun B siRNA (m) sc-35727 10 µM $258
Jun B (h)-PR sc-35726-PR 10 µM $23
Jun B (m)-PR sc-35727-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Jun B shRNA Plasmid (h) sc-35726-SH 20 µg $520
Jun B shRNA Plasmid (m) sc-35727-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Jun B shRNA (h) Lentiviral Particles sc-35726-V 200 µl $625
Jun B shRNA (m) Lentiviral Particles sc-35727-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NIH/3T3 nuclear extract sc-2138 1000 µg $143
KNRK nuclear extract sc-2141 1000 µg $143
KNRK + PMA nuclear extract sc-2142 1000 µg $143
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
Jun B (m): 293T Lysate sc-121169 100µg/200µl $205
Jun B (h): 293T Lysate sc-159648 100µg/200µl $205

Jun B Background Information
The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein, along with c-Fos, is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D, have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.

Jun B (C-11) Product Citations
See how others have used Jun B (C-11): sc-8051 antibody and or Jun B (C-11) antibody conjugates.


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Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
 
Jun B (C-11)
Click on image to enlarge
Jun B (C-11): sc-8051. Western blot analysis of Jun B expression in KNRK nuclear extract from PMA-treated cells.
Jun B (C-11): sc-8051. Immunofluorescence staining of methanol-fixed KNRK cells showing nuclear localization.
Jun B siRNA (h): sc-35726. Western blot analysis of Jun B expression in non-transfected control (A) and Jun B siRNA transfected (B) HeLa cells. Blot probed with Jun B (C-11): sc-8051. GAPDH (FL-335): sc-25778 used as specificity and loading control.
Jun B (C-11): sc-8051. Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nuclear staining of surface epithelial cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Jun B (C-11): sc-8051. Western blot analysis of Jun B expression in non-transfected: sc-117752 (A) and mouse Jun B transfected: sc-121169 (B) 293T whole cell lysates.
Jun B (C-11): sc-8051. Western blot analysis of Jun B expression in HeLa whole cell lysate.
Jun B (C-11): sc-8051. Western blot analysis of Jun B expression in non-transfected: sc-117752 (A) and human Jun B transfected: sc-159648 (B) 293T whole cell lysates.
Jun B (C-11): sc-8051. Western blot analysis of Jun B expression in non-transfected: sc-117752 (A) and human Jun B transfected: sc-170824 (B) 293T whole cell lysates.
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