 |
Histone H1 (AE-4) Antibody: sc-8030 |
| Datasheet |
|
- mouse monoclonal IgG2a; 200 µg/ml
- raised against leukemia biopsy cells of human origin
- recommended for detection of Histone H1 from a broad range of mammalian species by WB, IP, IF, IHC(P), FCM and ELISA
- fluorescein (Histone H1 (AE-4) FITC: sc-8030 FITC) and rhodamine (Histone H1 (AE-4) TRITC: sc-8030 TRITC) conjugates for immunofluorescence, 200 µg/ml
- phycoerythrin conjugate for intracellular FCM, Histone H1 (AE-4) PE: sc-8030 PE, 100 tests
- agarose conjugate for IP studies, Histone H1 (AE-4) AC: sc-8030 AC, 500 µg/0.25 ml agarose
- Alexa Fluor® 405 (Histone H1 (AE-4) Alexa Fluor® 405: sc-8030 AF405), Alexa Fluor® 488 (Histone H1 (AE-4) Alexa Fluor® 488: sc-8030 AF488) and Alexa Fluor® 647 (Histone H1 (AE-4) Alexa Fluor® 647: sc-8030 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
|
|
| |
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
Histone H1 Background Information
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
| Histone H1 (AE-4) Product Citations |
See how others have used Histone H1 (AE-4): sc-8030 antibody and or Histone H1 (AE-4) antibody conjugates.
75 total citations Loading citations.
|
| |
| Alexa Fluor® is a
trademark of Molecular Probes Inc., OR., USA |
|
| |
|
|
|
Histone H1 (AE-4)
Click on image to enlarge
|
|
Histone H1 (AE-4): sc-8030. Western blot analysis of Histone H1 expression in LNCaP whole cell lysate.
Histone H1 (AE-4) PE: sc-8030 PE. Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal mouse IgG 2a: sc-2867. Histone H1 (AE-4): sc-8030. Immunofluorescence staining of methanol-fixed A-431 cells (A) and immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue (B) showing nuclear localization.
Histone H1 siRNA (h): sc-29347. Western blot analysis of Histone H1 expression in non-transfected control (A) and Histone H1 siRNA transfected (B) HeLa cells. Blot probed with Histone H1 (AE-4): sc-8030. Actin (I-19): sc-1616 used as specificity and loading control. Histone H1 (AE-4) Alexa Fluor 488: sc-8030 AF488. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG 2a: sc-3891. Histone H1 (AE-4): sc-8030. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Histone H1 (AE-4) AF647: sc-8030 AF647. Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal mouse IgG 2a: sc-24637. |
|
Download
|
|
