Histone H1 Antibody (AE-4): sc-8030

Histone H1 Antibody (AE-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Histone H1 across a broad range of species by applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Histone H1 (AE-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Histone H1 plays a crucial role in the structural organization of chromatin, binding to linker DNA between nucleosomes, facilitating DNA compaction into higher-order structures essential for proper gene regulation and cellular function. Histone H1 is primarily located in the nucleus, contributing to chromatin fiber formation, thereby influencing processes such as DNA replication, repair, and transcription. Histone H1 is vital for maintaining chromatin integrity and regulating access to genetic material, critical for cellular processes and overall organismal development. Histone H1 undergoes various post-translational modifications that can alter DNA and protein interactions, further emphasizing significance in cellular dynamics and gene expression regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Histone H1 Antibody (AE-4) References:

1. Do protein motifs read the histone code?  |  de la Cruz, X., et al. 2005. Bioessays. 27: 164-75. PMID: 15666348
2. The dynamics of histone H1 function in chromatin.  |  Bustin, M., et al. 2005. Mol Cell. 17: 617-20. PMID: 15749012
3. Inducible covalent posttranslational modification of histone H3.  |  Bode, AM. and Dong, Z. 2005. Sci STKE. 2005: re4. PMID: 15855410
4. Regulation of histone synthesis and nucleosome assembly.  |  Gunjan, A., et al. 2005. Biochimie. 87: 625-35. PMID: 15989979
5. The diverse functions of histone lysine methylation.  |  Martin, C. and Zhang, Y. 2005. Nat Rev Mol Cell Biol. 6: 838-49. PMID: 16261189
6. Gene regulation by histone H1: new links to DNA methylation.  |  Rupp, RA. and Becker, PB. 2005. Cell. 123: 1178-9. PMID: 16377556
7. Histone post-translational modifications and the response to DNA double-strand breaks.  |  Wurtele, H. and Verreault, A. 2006. Curr Opin Cell Biol. 18: 137-44. PMID: 16487697
8. Histone modifications: signalling receptors and potential elements of a heritable epigenetic code.  |  Nightingale, KP., et al. 2006. Curr Opin Genet Dev. 16: 125-36. PMID: 16503131
9. Histone H3 variants and their potential role in indexing mammalian genomes: the 'H3 barcode hypothesis'.  |  Hake, SB. and Allis, CD. 2006. Proc Natl Acad Sci U S A. 103: 6428-35. PMID: 16571659
10. Histone H1 in gene expression and development.  |  Jordan, A. 2016. Biochim Biophys Acta. 1859: 429-30. PMID: 26772994
11. Histone H1 Post-Translational Modifications: Update and Future Perspectives.  |  Andrés, M., et al. 2020. Int J Mol Sci. 21: PMID: 32824860
12. Histone H1 kills MRSA.  |  Marsman, G., et al. 2024. Cell Rep. 43: 114969. PMID: 39546397

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Histone H1 Antibody (AE-4) has 367 citations in a variety of scientific publications.

Primary antibodies like Anti-Histone H1 Antibody (AE-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Histone H1 Antibody (AE-4) Specifications

Alternate Product Name: Histone H1

Product Clone Name: AE-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Histone H1

Epitope Species: human

Product Reactivity/Detection: Histone H1

Species Reactivity/Detection: broad species

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 367 citations in various scientific publications

Available Conjugates/Forms for Histone H1 Antibody (AE-4): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, PE: Phycoerythrin, TRITC: Rhodamine