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p-Bad (Ser 136) Antibody: sc-7999 |
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- goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-7999-R
- epitope corresponding to a short amino acid sequence containing phosphorylated Ser 136 of Bad of mouse origin
- recommended for detection of Ser 136 phosphorylated Bad of mouse origin and correspondingly phosphorylated Ser 137 of rat origin and Ser 99 phosphorylated Bad of human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine and bovine
- blocking peptide, sc-7999 P
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Bad Background Information
Phosphorylation of BAD, a pro-apoptotic member of the Bcl-2 protein family, on either serine 112 or serine 136 is thought to be necessary and sufficient for growth factors to promote cell survival (1–3). Serine 155 is a major site of phosphorylation by protein kinase A (PKA) and serum-induced kinases (1). Serine 155 phosphorylation requires the prior phosphorylation of serine 136, which recruits 14-3-3 proteins that then function to increase the accessibility of serine 155 to survival-promoting kinases (4). Like serine 112 and serine 136, phosphorylation of serine 155 inhibits the pro-apoptotic function of BAD (1). Serine 155 phosphorylation disrupts the binding of BAD to prosurvival Bcl-2 proteins and thereby promotes cell survival (4,5).
| p-Bad (Ser 136) Product Citations |
See how others have used p-Bad (Ser 136): sc-7999 antibody and or p-Bad (Ser 136) antibody conjugates.
12 total citations Loading citations.
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p-Bad (Ser 136)
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p-Bad (Ser 136)-R: sc-7999-R. Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A) and human Bad transfected: sc-170552 (B) 293T whole cell lysates. p-Bad (Ser 136)-R: sc-7999-R. Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A), untreated human Bad transfected: sc-170552 (B)and lambda protein phosphatase treated human Bad transfected: sc-170552 (C) 293T whole cell lysates. p-Bad (Ser 136)-R: sc-7999-R. Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A), untreated human Bad transfected: sc-170552 (B) and lambda protein phosphatase treated human Bad transfected: sc-170552 (C) 293T whole cell lysates. Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A, D), untreated human Bad transfected: sc-170552 (B, E) and lambda protein phosphatase treated human Bad transfected: sc-170552 (C, F) 293T whole cell lysates. Antibodies tested include p-Bad (Ser 136)-R: sc-7999-R (A, B, C) and Bad (H-168): sc-7869 (D, E, F). Western blot analysis of Bad phosphorylation in non-transfected: sc-117752 (A,E), untreated human Bad transfected: sc-170552 (B,F), PMA treated human Bad transfected: sc-170552 (C,G) and PMA and lambda protein phosphatase ( sc-200312A) treated human Bad transfected: sc-170552 (D,H) 293T whole cell lysates. Antibodies tested include p-Bad (Ser 136)-R: sc-7999-R (A,B,C,D) and Bad (H-168): sc-7869 (E,F,G,H). |
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