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p-MEK-1/2 (Ser 218/Ser 222) Antibody: sc-7995 |
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- goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-7995-R
- recommended for detection of MEK-1 dually phosphorylated at Ser 218 and Ser 222 and correspondingly phosphorylated MEK-2 of mouse, rat, human, Drosophila and Xenopus origin by WB, IP, IF and ELISA
- blocking peptide, sc-7995 P; also reactive with additional species, including equine, canine, bovine, porcine and avian
- agarose conjugate for IP studies, sc-7995 AC, 500 µg/0.25 ml agarose
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MEK-1/2 Background Information
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK (1). A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity (2). MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases (3). MEK-5 appears to specifically phosphorylate ERK5 (4), whereas MEK-6 phosphorylates p38 and p38ß (5,6). MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway (7–9). Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAFor MEKK1) positively regulates the kinase activity.
| p-MEK-1/2 (Ser 218/Ser 222) Product Citations |
See how others have used p-MEK-1/2 (Ser 218/Ser 222): sc-7995 antibody and or p-MEK-1/2 (Ser 218/Ser 222) antibody conjugates.
19 total citations Loading citations.
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p-MEK-1/2 (Ser 218/Ser 222)
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Western blot analysis of inactive mouse (A,C,E) and active His-tagged human (B,D,F) recombinant MEK-1. Antibodies tested include MEK-1 (H-8): sc-6250 (A,B), p-MEK-1/2 (Ser 218/Ser 222): sc-7995 (C,D), and p-MEK-1/2 (Ser 218/Ser 222)-R: sc-7995-R (E,F). p-MEK-1/2 (Ser 218/Ser 222): sc-7995. Immunofluorescence staining of methanol-fixed SCC-4 cells showing cytoplasmic localization.
p-MEK-1/2 (Ser 218/Ser 222): sc-7995. Western blot analysis of MEK-2 phosphorylation in non-transfected: sc-117752 (A) and human MEK-2 transfected: sc-158719 (B) 293T whole cell lysates. p-MEK-1/2 (Ser 218/Ser 222): sc-7995. Western blot analysis of MEK-1/2 phosphorylation in untreated (A), PMA treated (B) and PMA and lambda protein phosphatase treated (C) HeLa whole cell lysates.
Western blot analysis of MEK-1/2 phosphorylation in untreated (A,D), serum starved and PMA treated (B,E) and serum starved, PMA treated and lambda protein phosphatase ( sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-MEK-1/2 (Ser 218/Ser 222)-R: sc-7995-R (A,B,C) and MEK-1 (H-8): sc-6250 (D,E,F). |
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