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p-MEK-3/6 (Ser 189)-R Antibody: sc-7994-R |
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- rabbit polyclonal IgG, 200 µg/ml
- epitope corresponding to a short amino acid sequence containing phosphorylated Ser 189 of MEK-3 of human origin
- recommended for detection of Ser 189 phosphorylated MEK-3 and Ser 207 phosphorylated MEK-6 of mouse, rat, human, Drosophila, Xenopus and zebrafish origin by WB, IP, IF and ELISA
- blocking peptide, sc-7994 P
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MEK-3/6 Background Information
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK (1). A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity (2). MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases (3). MEK-5 appears to specifically phosphorylate ERK5 (4), whereas MEK-6 phosphorylates p38 and p38b (5,6). MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway (7-9).
| p-MEK-3/6 (Ser 189)-R Product Citations |
See how others have used p-MEK-3/6 (Ser 189)-R: sc-7994-R antibody and or p-MEK-3/6 (Ser 189)-R antibody conjugates.
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p-MEK-3/6 (Ser 189)-R
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Western blot analysis of l phosphatase-treated (A,C) and untreated (B,D) active human recombinant MEK-6. Antibodies tested include MEK-6 (N-19): sc-1992 (A,B) and p-MEK 3/6 (Ser-189)-R: sc-7994-R (C,D). p-MEK-3/6 (Ser 189)-R: sc-7994-R. Western blot analysis of MEK-6 phosphorylation in non-transfected: sc-117752 (A) and human MEK-6 transfected: sc-113820 (B) 293T whole cell lysates. p-MEK-3/6 (Ser 189)-R: sc-7994-R. Western blot analysis of MEK-3 phosphorylation in non-transfected: sc-117752 (A) and human MEK-3 transfected: sc-114954 (B) 293T whole cell lysates. p-MEK-3/6 (Ser 189)-R: sc-7994-R. Western blot analysis of MEK-6 phosphorylation in non-transfected: sc-117752 (A) and human MEK-6 transfected: sc-113820 (B) 293T whole cell lysates. Western blot analysis of l phosphatase-treated (A,C) and untreated (B,D) active human recombinant MEK-6. Antibodies tested include MEK-6 (N-19): sc-1992 (A,B) and p-MEK 3/6 (Ser-189)-R: sc-7994-R (C,D). p-MEK-3/6 (Ser 189)-R: sc-7994-R. Western blot analysis of MEK-3 phosphorylation in non-transfected: sc-117752 (A) and human MEK-3 transfected: sc-114954 (B) 293T whole cell lysates. Western blot analysis of MEK-3 phosphorylation in non-transfected: sc-117752 (A,D), untreated human MEK-3 transfected: sc-114954 (B,E) and lambda protein phosphatase ( sc-200312A) treated human MEK-3 transfected: sc-114954 (C,F) 293T whole cell lysates. Antibodies tested include p-MEK-3/6 (Ser 189)-R: sc-7994-R (A,B,C) and MEK-3/6 (H-90): sc-13069 (D,E,F). |
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