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home » Cell Cycle Proteins » Cdc Antibody / Cdc Antibodies » p-Cdc2 p34 (Tyr 15): sc-7989

p-Cdc2 p34 (Tyr 15) Antibody: sc-7989

  |  Datasheet
  • goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-7989-R
  • recommended for detection of Cdc2 of mouse, rat and human origin phosphorylated at Tyr 15 and correspondingly phosphorylated Cdk2 and Cdk3 by WB, IP, IF and ELISA; also reactive with additional mammalian species, including cow
  • blocking peptide, sc-7989 P; also reactive with additional species, including equine, canine, bovine, porcine and avian
 
Additional Cdc Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human CDC2 983 10q21.2 NM_001786, NM_033379 P06493
 116940
Mouse Cdc2a 12534 10 B5.3 P11440
 N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p-Cdc2 p34 (Tyr 15) sc-7989 200 µg/ml $279
p-Cdc2 p34 (Tyr 15) P sc-7989 P
(peptide)
 100 µg/0.5 ml $61
p-Cdc2 p34 (Tyr 15)-R sc-7989-R 200 µg/ml $279
Lambda Phosphatase sc-200312 100,000 units $462
Lambda Phosphatase sc-200312A 20,000 units $116
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Saos-2 + hydroxyurea Cell Lysate sc-2286 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
HeLa + hydroxyurea Cell Lysate sc-24682 500 µg/200 µl $104
NAMALWA Cell Lysate sc-2234 500 µg/200 µl $104
Cdc2 p34 (h5): 293T Lysate sc-158363 100µg/200µl $205

Cdc2 p34 Background Information
Cdc2, an evolutionarily conserved serine/threonine-specific protein kinase, is essential in the cell cycle transition from G2 to M phase. Cdc2 is regulated by association with B-type cyclins and by reversible phosophorylation. Cyclin B binding facilitates the phosphorylation of Cdc2 p34 on three regulatory sites: threonine 14, tyrosine 15, and threonine 161. In higher eukaryotes, Cdc2 is negatively regulated by phosphorylation of two residues located in the ATP-binding site, Thr 14 and Tyr 15. Cdc2 is positively regulated by the cyclin-dependent phosphorylation of Thr 161. Both phosphorylation and de- phosphorylation at Thr 161 are required for progression through the cell cycle.

p-Cdc2 p34 (Tyr 15) Product Citations
See how others have used p-Cdc2 p34 (Tyr 15): sc-7989 antibody and or p-Cdc2 p34 (Tyr 15) antibody conjugates.


8 total citations
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p-Cdc2 p34 (Tyr 15)
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sc-7989
Western blot detection of Cdc2 p34 phosphorylation using Saos-2 cells treated with hydroxyurea. Blots were probed with Cdc2 p34 (B-6): sc-8395 (A), p-Cdc2 p34 (Tyr 15)-R: sc-7989-R preincubated with cognate phosphorylated peptide (B) and p-Cdc2 p34 (Tyr 15)-R: sc-7989-R pre-incubated with identical unphosphorylated peptide (C).
sc-7989
p-Cdc2 p34 (Tyr 15): sc-7989. Western blot analysis of phosphorylated Cdc2 p34 expression in HeLa (A) and hydroxyurea-treated HeLa (B) whole cell lysates.
sc-7989
Western blot analysis of phosphorylated Cdc2 p34 expression in Saos-2 (A,C) and HeLa (B,D) whole cell lysates. Blots were probed with p-Cdc2 p34 (Tyr 15)-R: sc-7989-R (A,B) and p-Cdc2 p34 (Tyr 15)-R: sc-7989-R preincubated with its cognate phosphorylated peptide (C,D).
sc-7989
p-Cdc2 p34 (Tyr 15)-R: sc-7989-R. Western blot analysis of Cdc2 p34 phosphorylation in non-transfected 293: sc-110760 (A), human Cdc2 p34 transfected 293: sc-128282 (B) and HeLa (C) whole cell lysates.
sc-7989
p-Cdc2 p34 (Tyr 15): sc-7989. Western blot analysis of Cdk2 phosphorylation in non-transfected: sc-117752 (A) and mouse Cdk2 transfected: sc-119146 (B) 293T whole cell lysates.
sc-7989
Cdc2 p34 (Tyr 15): sc-7989. Western blot analysis of Cdc2 p34 expression in non-transfected 293: sc-110760 (A), human Cdc2 p34 transfected 293: sc-128282 (B) and HeLa (C) whole cell lysates.
sc-7989
p-Cdc2 p34 (Tyr 15)-R: sc-7989-R. Western blot analysis of Cdc2 p34 phosphorylation in non-transfected 293T: sc-117752 (A), mouse Cdc2 p34 transfected 293T: sc-119124 (B) and K-562 (C) whole cell lysates.
sc-7989
p-Cdc2 p34 (Tyr 15)-R: sc-7989-R. Western blot analysis of Cdc2 p34 phosphorylation in non-transfected 293T: sc-117752 (A), mouse Cdc2 p34 transfected 293T: sc-119124 (B) and K-562 (C) whole cell lysates.
sc-7989
p-Cdc2 p34 (Tyr 15): sc-7989. Western blot analysis of phosphorylated Cdc2 p34 expression in HeLa (A) and hydroxyurea-treated HeLa (B) whole cell lysates.
sc-7989
Western blot analysis of phosphorylated Cdc2 p34 expression in Saos-2 (A,C) and HeLa (B,D) whole cell lysates. Blots were probed with p-Cdc2 p34 (Tyr 15)-R: sc-7989-R (A,B) and p-Cdc2 p34 (Tyr 15)-R: sc-7989-R preincubated with its cognate phosphorylated peptide (C,D).
sc-7989
p-Cdc2 p34 (Tyr 15)-R: sc-7989-R. Western blot analysis of Cdc2 p34 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) HeLa whole cell lysates.
sc-7989
p-Cdc2 p34 (Tyr 15)-R: sc-7989-R. Western blot analysis of Cdc2 p34 phosphorylation in HeLa whole cell lysate.
sc-7989
p-Cdc2 p34 (Tyr 15): sc-7989. Western blot analysis of Cdc2 p34 phosphorylation in non-transfected: sc-117752 (A) and human Cdc2 p34 transfected: sc-158363 (B) 293T whole cell lysates.
sc-7989
Western blot analysis of Cdc2 p34 phosphorylation in untreated (A,F), Hydroxyurea treated (B,G), nocodazole treated (C,H), Hydroxyurea and lambda protein phosphatase treated (D,I) and nocodazole and lambda protein phosphatase treated (E,J) HeLa whole cell lysates. Antibodies tested include p-Cdc2 p34 (Tyr 15)-R: sc-7989-R (A-E) and p-Cdc2 p34 (PSTAIRE): sc-53 (F-J).
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