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home » Transcription Regulators » ATF Antibody / ATF Antibodies » p-ATF-2 (Thr 71): sc-7982

p-ATF-2 (Thr 71) Antibody: sc-7982

  |  Datasheet
  • goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-7982-R
  • recommended for detection of ATF-2 of mouse, rat, human and Xenopus origin phosphorylated at Thr 71 by WB, IP, IF, IHC(P) and ELISA
  • blocking peptide, sc-7982 P; also reactive with additional species, including equine, bovine, porcine and avian
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-7982 X, 200 µg/0.1 ml
 
Additional ATF Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   Gel Shift   ChIP   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human ATF2 1386 2q31.1 NM_001880 P15336
 123811
Mouse Atf2 11909 2 C3 P16951
 N/A
 
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p-ATF-2 (Thr 71) sc-7982 200 µg/ml $279
p-ATF-2 (Thr 71) P sc-7982 P
(peptide)
 100 µg/0.5 ml $61
p-ATF-2 (Thr 71) X sc-7982 X 200 µg/0.1 ml $279
p-ATF-2 (Thr 71)-R sc-7982-R 200 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ATF-2 siRNA (h) sc-29205 10 µM $258
ATF-2 siRNA (m) sc-29756 10 µM $258
ATF-2 (h)-PR sc-29205-PR 10 µM $23
ATF-2 (m)-PR sc-29756-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ATF-2 shRNA Plasmid (h) sc-29205-SH 20 µg $520
ATF-2 shRNA Plasmid (m) sc-29756-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ATF-2 shRNA (h) Lentiviral Particles sc-29205-V 200 µl $625
ATF-2 shRNA (m) Lentiviral Particles sc-29756-V 200 µl $625
 WB Positive Control Cell Lysate (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NIH/3T3 + anisomycin Cell Lysate sc-2247 500 µg/200 µl $104

ATF-2 Background Information
Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promotor and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro. The gene encoding human ATF-2 maps to chromosome 2q31.1.

p-ATF-2 (Thr 71) Product Citations
See how others have used p-ATF-2 (Thr 71): sc-7982 antibody and or p-ATF-2 (Thr 71) antibody conjugates.


6 total citations
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p-ATF-2 (Thr 71)
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sc-7982
p-ATF-2 (Thr 71)-R: sc-7982-R. Immunofluorescence staining of methanol-fixed, anisomycin-treated NIH/3T3 cells showing nuclear staining.
sc-7982
p-ATF-2 (Thr 71)-R: sc-7982-R. Western blot analysis of ATF-2 phosphorylation in anisomycin-treated NIH/3T3 cells. Antibody was preincubated with either the cognate phosphopeptide (A) or non-phosphorylated peptide (B).
sc-7982
Western blot analysis of human recombinant ATF-2 untreated fusion protein (A,C) or phosphorylated by human recombinant JNK1 (B,D). Antibodies tested include ATF-2 (N-96): sc-6233 (A,B) and p-ATF-2 (Thr 71)-R: sc-7982-R (C,D).
sc-7982
p-ATF-2 (Thr 71)-R: sc-7982-R. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization.
sc-7982
p-ATF-2 (Thr 71)-R: sc-7982-R. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization.
sc-7982
p-ATF-2 (Thr 71)-R: sc-7982-R. Immunofluorescence staining of methanol-fixed, anisomycin-treated NIH/3T3 cells showing nuclear staining.
sc-7982
p-ATF-2 (Thr 71)-R: sc-7982-R. Western blot analysis of ATF-2 phosphorylation in anisomycin-treated NIH/3T3 cells. Antibody was preincubated with either the cognate phosphopeptide (A) or non-phosphorylated peptide (B).
sc-7982
Western blot analysis of human recombinant ATF-2 untreated fusion protein (A,C) or phosphorylated by human recombinant JNK1 (B,D). Antibodies tested include ATF-2 (N-96): sc-6233 (A,B) and p-ATF-2 (Thr 71)-R: sc-7982-R (C,D).
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