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p-c-Jun (Ser 73) Antibody: sc-7981 |
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- goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-7981-R
- recommended for detection of Ser 73 phosphorylated c-Jun and correspondingly phosphorylated Ser 100 JunD of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA; also reactive with additional mammalian species, including pig
- blocking peptide, sc-7981 P; also reactive with additional species, including equine, canine, bovine, porcine and avian
- TransCruz reagent for Gel Supershift and ChIP applications, sc-7981 X, 200 µg/0.1 ml
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c-Jun Background Information
Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are found to be associated with a number of transcriptional complexes (1,2). The c-Jun protein is a major component of the transcription factor AP-1 (3,4), originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE) (3,4). The Jun proteins form homo- and heterodimers which bind the TRE, but the Fos proteins are active only as heterodimers with any of the Jun proteins (5,6). Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain (7). An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is deactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA) (8).
| p-c-Jun (Ser 73) Product Citations |
See how others have used p-c-Jun (Ser 73): sc-7981 antibody and or p-c-Jun (Ser 73) antibody conjugates.
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p-c-Jun (Ser 73)
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Western blot analysis of human recombinant c-Jun fusion protein (A,C) and human recombinant c-Jun fusion protein phosphorylated by human recombinant JNK1 (B,D). Antibodies tested include p-c-Jun (Ser 63/73)-R: sc-16312-R (A,B) and p-c-Jun (Ser 73)-R: sc-7981-R (C,D). Western blot analysis of non-phosphorylated human recombinant c-Jun fusion protein (A,C,E) phosphorylated by human recombinant JNK1 (B,D,F). Antibodies tested include c-Jun (H-79): sc-1694 (A,B), p-c-Jun (Ser 73)-R: sc-7981-R (C,D) and p-c-Jun (Ser 63/73)-R: sc-16312-R (E,F). p-c-Jun (Ser 73): sc-7981. Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125069 (B) 293T whole cell lysates. p-c-Jun (Ser 73)-R: sc-7981-R. Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125069 (B) 293T whole cell lysates. p-c-Jun (Ser 73)-R: sc-7981-R. Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125070 (B) 293T whole cell lysates. p-c-Jun (Ser 73)-R: sc-7981-R. Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125069 (B) 293T whole cell lysates. p-c-Jun (Ser 73)-R: sc-7981-R. Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125070 (B) 293T whole cell lysates. Western blot analysis of human recombinant c-Jun fusion protein (A,C) and human recombinant c-Jun fusion protein phosphorylated by human recombinant JNK1 (B,D). Antibodies tested include p-c-Jun (Ser 63/73)-R: sc-16312-R (A,B) and p-c-Jun (Ser 73)-R: sc-7981-R (C,D). Western blot analysis of non-phosphorylated human recombinant c-Jun fusion protein (A,C,E) phosphorylated by human recombinant JNK1 (B,D,F). Antibodies tested include c-Jun (H-79): sc-1694 (A,B), p-c-Jun (Ser 73)-R: sc-7981-R (C,D) and p-c-Jun (Ser 63/73)-R: sc-16312-R (E,F). p-c-Jun (Ser 73): sc-7981. Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125069 (B) 293T whole cell lysates. p-c-Jun (Ser 73)-R: sc-7981-R. Western blot analysis of c-Jun phosphorylation in non-transfected 293T: sc-117752 (A), mouse c-Jun transfected 293T: sc-125071 (B) and NIH/3T3 (C) whole cell lysates. Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A, D), untreated mouse c-Jun transfected: sc-125069 (B, E) and lambda protein phosphatase treated mouse c-Jun transfected: sc-125069 (C, F) 293T whole cell lysates. Antibodies tested include p-c-Jun (Ser 73)-R: sc-7981-R (A, B, C) and c-Jun (N): sc-45 (D, E, F). p-c-Jun (Ser 73)-R: sc-7981-R. Western blot analysis of c-Jun expression in non-transfected: sc-117752 (A) and human c-Jun transfected: sc-159456 (B) 293T whole cell lysates. Western blot analysis of c-Jun phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse c-Jun transfected: sc-125069 (B,E) and lambda protein phosphatase ( sc-200312A) treated human c-Jun transfected: sc-125069 (C,F) 293T whole cell lysates. Antibodies tested include p-c-Jun (Ser 73)-R: sc-7981-R (A,B,C) and c-Jun (H-79): sc-1694 (D,E,F). Western blot analysis of c-Jun phosphorylation in untreated (A,D), UV irradiated (B,E) and UV irradiated and lambda protein phosphatase ( sc-200312A) treated (C,F) NIH/3T3 whole cell lysates. Antibodies tested include p-c-Jun (Ser 73)-R: sc-7981-R (A,B,C) and c-Jun (H-79): sc-1694 (D,E,F). |
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