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- mouse monoclonal IgM; 200 µg/ml
- recommended for detection of Tyr 182 phosphorylated p38α, p38β and p38γ by WB, IP, IF, IHC(P) and ELISA
- blocking peptide, sc-7973 P; also reactive with additional species, including equine, canine, bovine, porcine and avian
- agarose conjugate for IP studies, sc-7973 AC, 500 µg/0.25 ml agarose
- fluorescein (sc-7973 FITC) and rhodamine (sc-7973 TRITC) conjugates for immunofluorescence, 200 µg/ml
- for phosphorylation positive (sc-2217) and non-activated control (sc-2210) cell extracts
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p38 Background Information
MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38å, p38∫ and p38©, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38å, p38∫ and p38© are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation. The p38 proteins are subject to phosphoryation on Thr and Tyr residues, an event which is thought to activate the phosphorylated protein.
| p-p38 (D-8) Product Citations |
See how others have used p-p38 (D-8): sc-7973 antibody and or p-p38 (D-8) antibody conjugates.
102 total citations Loading citations.
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p-p38 (D-8)
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p-p38 (D-8): sc-7973. Immunofluorescence staining of methanol-fixed NIH/3T3 cells (A) and immuno-peroxidase staining of formalin-fixed, paraffin-embedded human liver carcinoma tissue (B) showing nuclear localization of activated p38.
Western blot analysis of p38 activation in whole cell lysates prepared from control (A,C) and heat-shocked (B,D) NIH/3T3 cultures. Antibodies tested include p-p38 (D-8): sc-7973 (A,B) and p38 (C-20): sc-535 (C,D). p38 (H-147): sc-7973. Western blot analysis of p38α expression in non-transfected 293T: sc-117752 (A), mouse p38α transfected 293T: sc-122319 (B) and Jurkat (C) whole cell lysates. p-p38 (D-8): sc-7973. Western blot analysis of p38α phosphorylation expression in non-transfected: sc-117752 (A) and human p38α transfected: sc-114258 (B) 293T whole cell lysates. p-p38 (D-8): sc-7973. Immunofluorescence staining of methanol-fixed NIH/3T3 cells (A) and immuno-peroxidase staining of formalin-fixed, paraffin-embedded human liver carcinoma tissue (B) showing nuclear localization of activated p38.
Western blot analysis of p38α activation in whole cell lysates prepared from control (A,C) and heat-shocked (B,D) NIH/3T3 cultures. Antibodies tested include p-p38 (D-8): sc-7973 (A,B) and p38α (C-20): sc-535 (C,D). p-p38 (D-8): sc-7973. Immunofluorescence staining of methanol-fixed NIH/3T3 cells (A) and immuno-peroxidase staining of formalin-fixed, paraffin-embedded human liver carcinoma tissue (B) showing nuclear localization of activated p38.
p-p38 (D-8): sc-7973. Western blot analysis of p38α phosphorylation expression in non-transfected: sc-117752 (A) and human p38α transfected: sc-114258 (B) 293T whole cell lysates. p38 (H-147): sc-7973. Western blot analysis of p38α expression in non-transfected 293T: sc-117752 (A), mouse p38α transfected 293T: sc-122319 (B) and Jurkat (C) whole cell lysates. |
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