beta-catenin Antibody (E-5): sc-7963

β-catenin Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody specifically developed to target amino acids 680-781 at the C-terminus of human β-catenin. β-catenin monoclonal antibody (E-5) effectively detects β-catenin from mouse, rat, and human origins across various applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, anti-β-catenin antibody (E-5) offers versatile solutions for diverse experimental needs. β-Catenin, also known as catenin beta-1 or CTNNB1, is a multifunctional protein integral to cell-cell adhesion and gene transcription regulation. β-catenin binds to the intracellular cytoplasmic tail of cadherins, including E-cadherin, forming catenin-cadherin complexes that are essential for maintaining cellular adhesion and tissue architecture. Beyond its structural role, β-catenin serves as a key component of the Wnt signaling pathway, where accumulation in the cytoplasm and subsequent translocation to the nucleus influence the transcription of genes involved in cell proliferation and differentiation. Dysregulation of β-catenin has been implicated in various cancers, highlighting its importance in cellular signaling and adhesion. β-catenin monoclonal antibody (E-5) is an indispensable tool for researchers studying these critical cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

beta-catenin Antibody (E-5) References:

1. The many faces and functions of β-catenin.  |  Valenta, T., et al. 2012. EMBO J. 31: 2714-36. PMID: 22617422
2. Direct targeting of β-catenin in the Wnt signaling pathway: Current progress and perspectives.  |  Wang, Z., et al. 2021. Med Res Rev. 41: 2109-2129. PMID: 33475177
3. Wnt/β-catenin Signaling Inhibitors.  |  Zhang, X., et al. 2023. Curr Top Med Chem. 23: 880-896. PMID: 36872364
4. USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization.  |  Tang, J., et al. 2023. Cell Death Dis. 14: 360. PMID: 37311739
5. Genetic inactivation of β-catenin is salubrious, whereas its activation is deleterious in desmoplakin cardiomyopathy.  |  Olcum, M., et al. 2023. Cardiovasc Res. 119: 2712-2728. PMID: 37625794
6. Frequent alterations in E-cadherin and alpha- and beta-catenin expression in human breast cancer cell lines.  |  Pierceall, WE., et al. 1995. Oncogene. 11: 1319-26. PMID: 7478552
7. Role of the E-cadherin/alpha-catenin complex in modulating cell-cell and cell-matrix adhesive properties of invasive colon carcinoma cells.  |  Breen, E., et al. 1995. Ann Surg Oncol. 2: 378-85. PMID: 7496831
8. Receptor protein tyrosine phosphatase PTPmu associates with cadherins and catenins in vivo.  |  Brady-Kalnay, SM., et al. 1995. J Cell Biol. 130: 977-86. PMID: 7642713
9. Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin.  |  Sacco, PA., et al. 1995. J Biol Chem. 270: 20201-6. PMID: 7650039
10. Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.  |  Knudsen, KA., et al. 1995. J Cell Biol. 130: 67-77. PMID: 7790378
11. Beta-catenin expression in human cancers.  |  Takayama, T., et al. 1996. Am J Pathol. 148: 39-46. PMID: 8546224
12. Cloning of an alternative form of plakoglobin (gamma-catenin) lacking the fourth armadillo repeat.  |  Ozawa, M., et al. 1995. J Biochem. 118: 836-40. PMID: 8576101

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-beta-catenin Antibody (E-5) has 1,001 citations in a variety of scientific publications.

Primary antibodies like Anti-beta-catenin Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

beta-catenin Antibody (E-5) Specifications

Alternate Product Name: β-catenin

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 680-781

Epitope Target: β-catenin

Epitope Species: human

Product Reactivity/Detection: β-catenin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1,001 citations in various scientific publications

Available Conjugates/Forms for beta-catenin Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin