Welcome to Santa Cruz Biotechnology!
SCBT Home
Home » Transcription Regulators » Smad Antibodies » Smad1/2/3 Antibody (H-2): sc-7960

Smad1/2/3 Antibody (H-2): sc-7960

 |  Datasheet

(Based on data analysis)

  • Smad1/2/3 Antibody (H-2) is a mouse monoclonal IgG2a provided at 200 µg/ml
  • raised against amino acids 1-465 representing full length Smad1 of human origin
  • recommended for detection of Smad1, Smad2, Smad3 and to a lesser extent, Smad 5 of mouse, human and mink origin by WB, IP, PAR, ELISA
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-7960 X, 200 µg/0.1 ml
  • See 31 citations for Smad1/2/3 Antibody (H-2)
 
Full-length Smad1/2/3 protein sequence with immunogen highlighted:
MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMTQDGSQPMDTNMMAPPLPSEINRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSV

See additional Smad Antibodies including Smad, Smad1, Smad2, Smad3, Smad4, Smad5, Smad6, Smad7 and Smad8.

Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   Gel Shift   ChIP  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
Smad1/2/3 Antibody (H-2) sc-7960 200 µg/ml $279
Smad1/2/3 Antibody (H-2) X sc-7960 X 200 µg/0.1 ml $279
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
Mv 1 Lu Cell Lysate sc-3810 500 µg/200 µl $104
Mv 1 Lu + TGF Cell Lysate sc-24737 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human SMAD1 4086 4q31.22 NM_001003688, NM_005900 Q15797
 601595
Mouse Smad1 17125 8 C2 P70340
 N/A
Mouse Smad2 17126 18 E3 Q62432
 N/A
Mouse Smad3 17127 9 C Q8BUN5
 N/A
 

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), have been implicated as downstream effectors of TGFß/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP2 and BMP4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFß and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFß signaling by interfering with TGF∫-mediated phosphorylation of other Smad family members.

Smad1/2/3 Antibody (H-2) Product Citations
See how others have used Smad1/2/3 Antibody (H-2) and or Smad1/2/3 Antibody (H-2) conjugates.


31 total citations
Loading citations.
 
Smad1/2/3 Antibody (H-2) Data
Click on image to enlarge
sc-7960
Smad1/2/3 (H-2): sc-7960. Western blot analysis of Smad expression in COS cells transfected with Smad1 (A), Smad2 (B), Smad3 (C), Smad4 (D), Smad5 (E), Smad6 (F) and Smad7 (G) expression vectors and empty vector (H).
sc-7960
Western blot analysis of Smad 2/3 phosphorylation in untreated (A,C) and TGFβ-treated Mv 1 Lu (B,D) whole cell lysates. Antibodies tested include Smad1/2/3 (H-2): sc-7960 (A,B) and p-Smad2/3 (Ser 433/435)-R (C,D): sc-11769-R.
sc-7960
Smad1/2/3 (H-2): sc-7960. Western blot analysis of Smad1/2/3 expression in Mv 1 Lu (A) and NIH/3T3 (B) whole cell lysates.
sc-7960
Smad1/2/3 (H-2): sc-7960. Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.
Download
Datasheets
Get Adobe Acrobat Reader