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- rabbit polyclonal IgG, 200 µg/ml
- epitope corresponding to amino acids 1-261 representing full length human PCNA
- recommended for detection of PCNA of mouse, rat, human, insect, zebrafish, Drosophila and Xenopus origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- agarose conjugate for IP studies, sc-7907 AC, 500 µg/0.25 ml agarose
- HRP conjugate, sc-7907 HRP, 200 µg/ml
- fluorescein (sc-7907 FITC) and rhodamine (sc-7907 TRITC) conjugates for immunofluorescence, 200 µg/ml
- Alexa Fluor® 405 (sc-7907 AF405), Alexa Fluor® 488 (sc-7907 AF488) and Alexa Fluor® 647 (sc-7907 AF647) conjugates for immunofluorescence; 100 µg/2 ml
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PCNA Background Information
The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.
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PCNA (FL-261)
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PCNA (FL-261): sc-7907. Western blot analysis of PCNA expression in A-431 (A) and NIH/3T3 (B) whole cell lysates.
PCNA (FL-261): sc-7907. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma showing nuclear localization (A). Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization (B).
PCNA (FL-261): sc-7907. Western blot analysis of PCNA expression in non-transfected 293T: sc-117752 (A), mouse PCNA transfected 293T: sc-127307 (B) and A-431 (C) whole cell lysates. PCNA (FL-261): sc-7907. Western blot analysis of PCNA expression in A-431 (A) and NIH/3T3 (B) whole cell lysates.
PCNA (FL-261): sc-7907. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma showing nuclear localization (A). Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization (B).
PCNA (FL-261): sc-7907. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma showing nuclear localization (A). Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization (B).
PCNA (FL-261): sc-7907. Western blot analysis of PCNA expression in non-transfected 293T: sc-117752 (A), mouse PCNA transfected 293T: sc-127307 (B) and A-431 (C) whole cell lysates. PCNA (FL-261): sc-7907. Western blot analysis of PCNA expression in non-transfected: sc-117752 (A) and human PCNA transfected: sc-159920 (B) 293T whole cell lysates. PCNA (FL-261): sc-7907. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in seminiferous ducts.
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