epitope corresponding to amino acids 1-197 representing full length p27 of mouse origin
recommended for detection of p27 of mouse, rat, human and mink origin by WB, IP, IF and ELISA; partially cross-reactive with the related mitotic inhibitory protein, p21
p27 Background Information Cell cycle progression is regulated by a series of cyclin-dependent kinases consisting of catalytic subunits, designated Cdks, as well as activating subunits, designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has recently been described that function as “mitotic inhibitors.” These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner; p16; and a more recently described p16-related inhibitor designated p15. A p21-related protein, p27, has been described as a negative regulator of G1 progression and speculated to function as a possible mediator of TGF∫-induced G1 arrest. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and, to a lesser extent, with cyclin E and Cdk2.
p27 (M-197) Product Citations
See how others have used p27 (M-197): sc-776 antibody and or p27 (M-197) antibody conjugates.
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p27 (M-197)
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Western blot analysis of p27 expression in Jurkat (A) and NIH/3T3 (B) whole cell lysates. Antibodies used include: p27 (M-197): sc-776 (A) and p27 (N-20): sc-527 (B).
p27 (M-197): sc-776. Western blot analysis of p27 expression in non-transfected: sc-110760 (A) and human p27 transfected: sc-110470 (B) 293 whole cell lysates.
p27 (M-197): sc-776. Western blot analysis of p27 expression in non-transfected: sc-117752 (A) and mouse p27 transfected: sc-122312 (B) 293T whole cell lysates.
p27 (M-197): sc-776. Western blot analysis of p27 expression in non-transfected: sc-110760 (A) and human p27 transfected: sc-129376 (B) 293 whole cell lysates.
