epitope mapping at the N-terminus of ARF of human origin
recommended for detection of ARF family GTP binding proteins of mouse, rat and human origin by ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
ARF Background Information The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins (1-3). ARFs influence vesicle trafficking and signal transduction in eukaryotic cells (1-3). ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of actin to the golgi via rho family-dependent G-protein localization (Rac, CDC42) and WASP/Arp2/3 complexes (3-7). Additionally, ARFs play a central role in maintenance of organelle integrity, assembly of coat proteins, and activation of phospholipase D (5-7). The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6); members of each class share a common gene organization (8,9). The human ARF1 gene maps to chromosome 1q42, contains five exons and four introns, and encodes a 181 amino acid protein (8,9).
