Nup35 siRNA (h), shRNA and Lentiviral Particle Gene Silencers

• 20 µg, up to 20 transfections
• shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
• shRNA sequences correspond to Nup35 siRNA Gene Silencer sequences
• provided as transfection-ready purified plasmid DNA target-specific gene silencing
• after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
• shRNA Plasmids are intended only for non-commercial use. Click here for details.
• suitable control antibody: contact Technical Service for availability of control antibody
• sqRT-PCR Primer also available: Nup35 (h)-PR: sc-75977-PR, 20 µl

• 200 µl viral stock containing 1x 10⁶ infectious units of virus (IFU) , sufficient for 10-20 transductions
• Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
• Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
• shRNA sequences correspond to Nup35 siRNA siRNA Gene Silencer sequences
• After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
• Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. Click here for details.
• Lentiviral Particles are intended for non-commercial use. Click here for details.