 |
- rabbit polyclonal IgG, 200 µg/ml
- epitope corresponding to amino acids 1-164 representing full length p21 of human origin
- recommended for detection of p21 of mouse, rat and human origin by WB, IP, IF and ELISA; partially cross-reactive with the related mitotic inhibitory protein, p27
|
|
| |
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
p21 Background Information
It is now well established that cyclins play a positive role in promoting cell cycle transitions via their ability to associate with and activate their cognate cyclin-dependent kinases (Cdks). Cdk2 associates with cyclins A, D and E, and has been implicated in the control of the G1 to S phase transition in mammals. A novel Cdk-interacting protein, designated p21, Cip1 or WAF1, has been identified in cyclin A, cyclin D1, cyclin E and Cdk2 immunoprecipitates. p21 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk 2, cyclin E-Cdk2, cyclin D1-Cdk4 and cyclin D2-Cdk4 complexes. Expression of p21 is inducible by wildtype, but not mutant, p53. The mouse homolog of p21 is designated CAP20.
| p21 (H-164) Product Citations |
See how others have used p21 (H-164): sc-756 antibody and or p21 (H-164) antibody conjugates.
72 total citations Loading citations.
|
| |
|
|
|
p21 (H-164)
Click on image to enlarge
|
Western blot analysis of p21 expression in C32 nuclear extracts (A,B). Antibodies tested include p21 (H-164): sc-756 (A) and p21 (C-19)-G: sc-397-G (B). p21 (H-164): sc-756. Immunofluorescence staining of methanol-fixed C32 cells showing nuclear staining.
p21 (H-164): sc-756. Western blot analysis of p21 expression in HeLa nuclear extract.
p21 (H-164): sc-756. Western blot analysis of p21 expression in untreated (A) and Novobiocin Sodium (U-6591) ( sc-358734) treated (B) T-47D whole cell lysates. Note down regulation of p21 expression in lane B. |
|
Download
|
|
