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- mouse monoclonal IgG2b; 200 µg/ml
- raised against amino acids 1-171 of Baxα of mouse origin
- recommended for detection of Baxα and Baxβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
- agarose conjugate for IP studies, sc-7480 AC, 500 µg/0.25 ml agarose
- fluorescein (sc-7480 FITC) and rhodamine (sc-7480 TRITC) conjugates for immunofluorescence, 200 µg/ml
- phycoerythrin (sc-7480 PE), PerCP (sc-7480 PerCP) and PerCP-Cy5.5 (sc-7480 PCPC5) conjugates for intracellular FCM, 100 tests
- Alexa Fluor® 405 (sc-7480 AF405), Alexa Fluor® 488 (sc-7480 AF488) and Alexa Fluor® 647 (sc-7480 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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Bax Background Information
The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax (Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activity of Bcl-2.
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See how others have used Bax (B-9): sc-7480 antibody and or Bax (B-9) antibody conjugates.
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Bax (B-9)
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Bax (B-9): sc-7480. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing membrane and cytoplasmic localization.
Western blot analysis of Bax expression in Jurkat (A), HuT 78 (B), Ramos (C) and BJAB (D) whole cell lysates. Antibodies tested include Bax (P-19): sc-526 (A,B) and Bax (B-9): sc-7480 (C,D). Bax (B-9) AF488: sc-7480 AF488. Intracellular FCM analysis of fixed and permeabilized CCRF-CEM cells. Black line histogram represents the isotype control, normal mouse IgG 2b: sc-3892. Bax (B-9): sc-7480. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubuli. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Bax (B-9): sc-7480. Western blot analysis of Bax expression in HuT 78 whole cell lysate.
Bax (B-9) FITC: sc-7480 FITC. Intracellular FCM analysis of fixed and permeabilized CCRF-CEM cells. Black line histogram represents the isotype control, normal mouse IgG 2b: sc-2857. Bax (B-9) AF647: sc-7480 AF647. Intracellular FCM analysis of fixed and permeabilized CCRF-CEM cells. Black line histogram represents the isotype control, normal mouse IgG 2b: sc-24638. Bax (B-9) PE: sc-7480 PE. Intracellular FCM analysis of fixed and permeabilized Ramos cells. Black line histogram represents the isotype control, normal mouse IgG 2b: sc-2868. Bax (B-9): sc-7480. Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tumor showing cytoplasmic localization.
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