Enolase (C-19) Antibody sc-7455

santa cruz biotechnology, inc.

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Enolase (C-19) Antibody: sc-7455	\| Datasheet

goat polyclonal IgG, 200 µg/ml
epitope mapping near the C-terminus of α Enolase of human origin
recommended for detection of α Enolase, β Enolase and γ Enolase of mouse, rat, human and yeast origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
blocking peptide, sc-7455 P
fluorescein (sc-7455 FITC) and rhodamine (sc-7455 TRITC) conjugates for immunofluorescence, 200 µg/ml
Alexa Fluor® 405 (sc-7455 AF405), Alexa Fluor® 488 (sc-7455 AF488) and Alexa Fluor® 647 (sc-7455 AF647) conjugates for immunofluorescence; 100 µg/2 ml

Additional Enolase Antibodies ...

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WB IP IF

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number

Human	ENO1	2023	1p36.23	NM_001428	P06733	n/a
Human	ENO2	2026	12p13.31	NM_001975	P09104	131360
Human	ENO3	2027	17p13.2	NM_001976, NM_053013	P13929	131370
Mouse	Eno1	13806	4 E2		P17182	N/A
Mouse	Eno2	13807	6 F2		P17183	N/A
Mouse	Eno3	13808	11 B3		P21550	N/A

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Ordering Information

Product Name	Catalog #	Unit	Price
Enolase (C-19)	sc-7455	200 µg/ml	$279
Enolase (C-19) Alexa Fluor® 405	sc-7455 AF405	100 µg/2 ml	$314
Enolase (C-19) Alexa Fluor® 488	sc-7455 AF488	100 µg/2 ml	$314
Enolase (C-19) Alexa Fluor® 647	sc-7455 AF647	100 µg/2 ml	$314
Enolase (C-19) FITC	sc-7455 FITC	200 µg/ml	$282
Enolase (C-19) P	sc-7455 P (peptide)	100 µg/0.5 ml	$61
Enolase (C-19) TRITC	sc-7455 TRITC	200 µg/ml	$282

WB Positive Control Cell Lysates (click product name for more information)

Product Name	Catalog #	Unit	Price
α Enolase (h): 293 Lysate	sc-112734	100µg/200µl	$205
Hep G2 Cell Lysate	sc-2227	500 µg/200 µl	$104
A549 Cell Lysate	sc-2413	500 µg/200 µl	$104
γ Enolase (m): 293T Lysate	sc-120040	100µg/200µl	$205
α Enolase (m): 293T Lysate	sc-124897	100µg/200µl	$205
α Enolase (h3): 293T Lysate	sc-170218	100µg/200µl	$205
γ Enolase (h): 293T Lysate	sc-170262	100µg/200µl	$205

Enolase Background Information
Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, å enolase, ∫ enolase and © enolase. å enolase expression has been detected on most tissues, whereas ∫ enolase is expressed predominantly in muscle tissue and © enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. The 433 amino acid protein shows 67% homology to yeast enolase and 94% homology to rat nonneural enolase. Studies also indicate that å enolase is encoded by the same gene that encodes tau-crystallin, a lens structural protein.

Enolase (C-19) Product Citations

See how others have used Enolase (C-19): sc-7455 antibody and or Enolase (C-19) antibody conjugates.

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Enolase (C-19)

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Enolase (C-19): sc-7455. Western blot analysis of Enolase expression in IMR-32 (A), Hep G2 (B), A549 (C), U-937 (D), NIH/3T3 (E) and KNRK (F) whole cell lysates and rat brain (G) and rat skeletal muscle (H) extracts.

α Enolase siRNA (h): sc-35310. Western blot analysis of α Enolase expression in non-transfected control (A) and α Enolase siRNA transfected (B) HeLa cells. Blot probed with Enolase (C-19): sc-7455. Actin (I-19): sc-1616 used as specificity and loading control.

Enolase (C-19): sc-7455. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.

Enolase (C-19): sc-7455. Western blot analysis of Enolase expression in non-transfected: sc-110760 (A) and human Enolase transfected: sc-112734 (B) 293 whole cell lysates.

Enolase (C-19): sc-7455. Western blot analysis of γ Enolase expression in non-transfected 293T: sc-117752 (A), mouse γ Enolase transfected 293T: sc-120040 (B) and Hep G2 (C) whole cell lysates.

Enolase (C-19): sc-7455. Western blot analysis of α Enolase expression in non-transfected: sc-117752 (A) and mouse α Enolase transfected: sc-124897 (B) 293T whole cell lysates.

Enolase (C-19): sc-7455. Western blot analysis of α Enolase expression in non-transfected: sc-117752 (A) and mouse α Enolase transfected: sc-124902 (B) 293T whole cell lysates.

Enolase (C-19): sc-7455. Western blot analysis of Enolase expression in HeLa whole cell lysate.

Enolase (C-19): sc-7455. Western blot analysis of γ Enolase expression in non-transfected 293T: sc-117752 (A), human γ Enolase transfected 293T: sc-170262 (B) and Y79 (C) whole cell lysates.

Enolase (C-19): sc-7455. Western blot analysis of Enolase expression in A549 whole cell lysate.

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Enolase (C-19) Antibody: sc-7455