Geminin Antibody (A-3): sc-74496

Geminin Antibody (A-3) is a mouse monoclonal IgG1 antibody that detects geminin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Geminin is a crucial nuclear protein that plays a vital role in regulating DNA replication initiation during the cell cycle. Anti-geminin antibody (A-3) functions by inhibiting the assembly of minichromosome maintenance (MCM) proteins with chromatin, thereby preventing the formation of the prereplication complex essential for DNA replication. Geminin levels fluctuate throughout the cell cycle, being lowest during the G1 phase and rising during the S, G2, and M phases, before decreasing again during mitosis. This cyclical expression is critical, as geminin degradation during mitosis, mediated by the anaphase-promoting complex (APC), allows for DNA replication initiation in the subsequent G1 phase. APC specifically targets proteins with a destruction box motif, including B-type cyclins, for degradation via ubiquitin-mediated proteolysis, highlighting geminin′s regulation importance in maintaining genomic stability and preventing untimely DNA replication. Geminin monoclonal antibody (A-3) is an essential tool for researchers studying cell cycle dynamics and DNA replication mechanisms.

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Geminin Antibody (A-3) References:

1. The regulation of embryonic patterning and DNA replication by geminin.  |  Pitulescu, M., et al. 2005. Cell Mol Life Sci. 62: 1425-33. PMID: 15870955
2. Geminin's double life: chromatin connections that regulate transcription at the transition from proliferation to differentiation.  |  Seo, S. and Kroll, KL. 2006. Cell Cycle. 5: 374-9. PMID: 16479171
3. Geminin is partially localized to the centrosome and plays a role in proper centrosome duplication.  |  Lu, F., et al. 2009. Biol Cell. 101: 273-85. PMID: 18798731
4. Role for Geminin in sustaining the activity of hematopoietic stem cells.  |  Takihara, Y. 2011. Cell Cycle. 10: 561-2. PMID: 21293183
5. Transcription of the Geminin gene is regulated by a negative-feedback loop.  |  Ohno, Y., et al. 2014. Mol Biol Cell. 25: 1374-83. PMID: 24554762
6. Geminin loss causes neural tube defects through disrupted progenitor specification and neuronal differentiation.  |  Patterson, ES., et al. 2014. Dev Biol. 393: 44-56. PMID: 24995796
7. Geminin facilitates FoxO3 deacetylation to promote breast cancer cell metastasis.  |  Zhang, L., et al. 2017. J Clin Invest. 127: 2159-2175. PMID: 28436938
8. Geminin ablation in vivo enhances tumorigenesis through increased genomic instability.  |  Champeris Tsaniras, S., et al. 2018. J Pathol. 246: 134-140. PMID: 29952003
9. Regulation of geminin by neuropeptide Y in vascular smooth muscle cell proliferation: A current review.  |  Liang, SY., et al. 2019. Herz. 44: 712-716. PMID: 30151710
10. Cas9-Geminin and Cdt1-fused anti-CRISPR protein synergistically increase editing accuracy.  |  Matsumoto, D., et al. 2023. FEBS Lett. 597: 985-994. PMID: 36905332

The preferred Geminin antibody is Geminin Antibody (F-7): sc-74456.