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Ribosomal Protein S6 Antibody (C-8): sc-74459

 |  Datasheet

(Based on data analysis)

  • Ribosomal Protein S6 Antibody (C-8) is a mouse monoclonal IgG2b provided at 200 µg/ml
  • raised against amino acids 1-249 mapping at the N-terminus of Ribosomal Protein S6 of human origin
  • recommended for detection of 40S ribosomal protein S6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • See 5 citations for Ribosomal Protein S6 Antibody (C-8)
 
Full-length Ribosomal Protein S6 protein sequence with immunogen highlighted:
MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQ

See additional Ribosomal Protein Antibodies including Ribosomal Protein, Ribosomal Protein LP0, Ribosomal Protein L10L, Ribosomal Protein L31, Ribosomal Protein L34, Ribosomal Protein L41, Ribosomal Protein L7L1, Ribosomal Protein LP1, Ribosomal Protein LP2, Ribosomal Protein S11, Ribosomal Protein S12, Ribosomal Protein S15, Ribosomal Protein S24, Ribosomal Protein S28, Ribosomal Protein S8, Ribosomal Protein S2, Ribosomal Protein L3, Ribosomal Protein S3, Ribosomal Protein S3A, Ribosomal Protein L4 , Ribosomal Protein L5, Ribosomal Protein S5, Ribosomal Protein L6, Ribosomal Protein S6, Ribosomal Protein L7, Ribosomal Protein L7A, Ribosomal Protein S7 , Ribosomal Protein L8, Ribosomal Protein L9 , Ribosomal Protein S9, Ribosomal Protein L10a , Ribosomal Protein S10, Ribosomal Protein L11 , Ribosomal Protein L12, Ribosomal Protein L13 , Ribosomal Protein S13, Ribosomal Protein L14, Ribosomal Protein S14, Ribosomal Protein L15 , Ribosomal Protein S15a, Ribosomal Protein S16, Ribosomal Protein L17, Ribosomal Protein S17, Ribosomal Protein L18, Ribosomal Protein S18, Ribosomal Protein L19, Ribosomal Protein S19, Ribosomal Protein S20, Ribosomal Protein L21, Ribosomal Protein S21, Ribosomal Protein L22, Ribosomal Protein L23, Ribosomal Protein L23a, Ribosomal Protein S23, Ribosomal Protein L24, Ribosomal Protein S25, Ribosomal Protein L26, Ribosomal Protein S26, Ribosomal Protein L27, Ribosomal Protein S27, Ribosomal Protein S27L, Ribosomal Protein L28, Ribosomal Protein L29, Ribosomal Protein S29, Ribosomal Protein L30, Ribosomal Protein L32, Ribosomal Protein L35, Ribosomal Protein L35A, Ribosomal Protein L36, Ribosomal Protein L36a, Ribosomal Protein L39, Ribosomal Protein L39L, Ribosomal Protein L22L1 and Ribosomal Protein L26L1.

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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
Ribosomal Protein S6 Antibody (C-8) sc-74459 200 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Ribosomal Protein S6 siRNA (h) sc-36424 10 µM $258
Ribosomal Protein S6 siRNA (m) sc-36425 10 µM $258
Ribosomal Protein S6 (h)-PR sc-36424-PR 10 µM, 20 µl $23
Ribosomal Protein S6 (m)-PR sc-36425-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Ribosomal Protein S6 shRNA Plasmid (h) sc-36424-SH 20 µg $520
Ribosomal Protein S6 shRNA Plasmid (m) sc-36425-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Ribosomal Protein S6 shRNA (h) Lentiviral Particles sc-36424-V 200 µl $625
Ribosomal Protein S6 shRNA (m) Lentiviral Particles sc-36425-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
KNRK Whole Cell Lysate sc-2214 500 µg/200 µl $104
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
Hep G2 Cell Lysate sc-2227 500 µg/200 µl $104
Ribosomal Protein S6 (m): 293T Lysate sc-125921 100 µg/200 µl $205
HL-60 Whole Cell Lysate sc-2209 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human RPS6 6194 9p22.1 NM_001010 P62753
 180460
Mouse Rps6 20104 4 C4 P62754
 N/A
 

The genes encoding for mammalian ribosomal proteins comprise multigene families that consist predominantly of multiple processed pseudogenes and one functional intro-containing gene within their coding regions (1). The rpS6 gene gives rise to Ribosomal protein S6 (also designated RPS6) (2). RPS6 is the major substrate of protein kinases in eukaryotic ribosomes (3-5). Sequence comparison has identified RPS6 as the equivalent of the ribosomal protein S10 from Saccharomyces cerevisiae (2). The sequence comparison of ribosomal proteins from evolutionarily distant eukaryotes, such as yeast and human, indicates that the structure and probably the function of RPS6 has been highly conserved (5).

Ribosomal Protein S6 Antibody (C-8) Product Citations
See how others have used Ribosomal Protein S6 Antibody (C-8) and or Ribosomal Protein S6 Antibody (C-8) conjugates.


5 total citations
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Ribosomal Protein S6 Antibody (C-8) Data
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sc-74459
Ribosomal Protein S6 (C-8): sc-74459. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
sc-74459
Ribosomal Protein S6 (C-8): sc-74459. Western blot analysis of Ribosomal Protein S6 expression in Hep G2 (A), K-562 (B), NIH/3T3 (C) and KNRK (D) whole cell lysates.
sc-74459
Ribosomal Protein S6 (C-8): sc-74459. Western blot analysis of Ribosomal Protein S6 expression in non-transfected 293T: sc-117752 (A), mouse Ribosomal Protein S6 transfected 293T: sc-125921 (B) and K-562 (C) whole cell lysates.
sc-74459
Ribosomal Protein S6 (C-8): sc-74459. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
sc-74459
Western blot analysis of Ribosomal Protein S6 phosphorylation in untreated (A,D), serum starved, EGF treated and serum treated (B,E) and serum starved, EGF treated, serum treated and lambda protein phosphatase (sc-200312A) treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-Ribosomal Protein S6 (Ser 236): sc-54279 (A,B,C) and Ribosomal Protein S6 (C-8): sc-74459 (D,E,F).
sc-74459
Western blot analysis of Ribosomal Protein S6 phosphorylation in untreated (A,D), serum starved, EGF treated and serum treated (B,E) and serum starved, EGF treated, serum treated and lambda protein phosphatase (sc-200312A) treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-Ribosomal Protein S6 (Ser 235): sc-101793 (A,B,C) and Ribosomal Protein S6 (C-8): sc-74459 (D,E,F).
sc-74459
Ribosomal Protein S6 (C-8): sc-74459. Immunoperoxi-dase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal and glial cells.
sc-74459
Western blot analysis of Ribosomal Protein S6 phosphorylation in untreated (A,D), EGF treated (B,E) and EGF and lambda protein phosphatase (sc-200312A) treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-Ribosomal Protein S6 (50.Ser 235/236): sc-293144 (A,B,C) and Ribosomal Protein S6 (C-8): sc-74459 (D,E,F).
sc-74459
Western blot analysis of Ribosomal Protein S6 phosphorylation in untreated (A,D), EGF treated (B,E) and EGF and lambda protein phosphatase (sc-200312A) treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-Ribosomal Protein S6 (51.Ser 244): sc-293145 (A,B,C) and Ribosomal Protein S6 (C-8): sc-74459 (D,E,F).
sc-74459
Western blot analysis of Ribosomal Protein S6 phosphorylation in untreated (A,D), EGF treated (B,E) and EGF and lambda protein phosphatase (sc-200312A) treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-Ribosomal Protein S6 (24.Ser 240): sc-293143 (A,B,C) and Ribosomal Protein S6 (C-8): sc-74459 (D,E,F).
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