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NF-1 Antibody (A-12): sc-74445

 |  Datasheet

(Based on data analysis)

  • NF-1 Antibody (A-12) is a mouse monoclonal IgG1 provided at 200 µg/ml
  • raised against amino acids 1-300 of NF-1 of human origin
  • recommended for detection of all NF-1 isoforms of mouse and human origin by WB, IP, IF, IHC(P) and ELISA
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-74445 X, 200 µg/0.1 ml
 

See additional NF-1 Antibodies including NF-1, NF-1, NF-1A and NF-1C.

Ordering InformationGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   Gel Shift   ChIP  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
NF-1 Antibody (A-12) sc-74445 200 µg/ml $279
NF-1 Antibody (A-12) X sc-74445 X 200 µg/0.1 ml $279
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
NIH/3T3 + PMA nuclear extract sc-2125 250 µg/0.05 ml $143
HeLa + PMA nuclear extract sc-2121 250 µg/0.05 ml $143
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
HeLa + PMA Cell Lysate sc-2258 500 µg/200 µl $104
NF-1 (h5): 293T Lysate sc-159124 100 µg/200 µl $205
NIH/3T3 nuclear extract sc-2138 250 µg/0.05 ml $143
Jurkat nuclear extract sc-2132 250 µg/0.05 ml $143
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human NFIC 4782 19p13.3 NM_005597, NM_205843 P08651
 600729
Mouse Nfia 18027 4 C6 Q02780
 N/A
Mouse Nfib 18028 4 C3 P97863
 N/A
Mouse Nfic 18029 10 C1 P70255
 N/A
Mouse Nfix 18032 8 C3 P70257
 N/A
 

NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, å-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.

NF-1 Antibody (A-12) Data
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sc-74445
NF-1 (A-12): sc-74445. Western blot analysis of NF-1 isoform expression in HeLa (A) and NIH/3T3 (B) nuclear extracts.
sc-74445
NF-1 (A-12): sc-74445. Western blot analysis of NF-1 expression in Jurkat whole cell lysate.
sc-74445
NF-1 (A-12): sc-74445. Western blot analysis of NF-1 expression in non-transfected: sc-117752 (A) and human NF-1 transfected: sc-159124 (B) 293T whole cell lysates.
sc-74445
NF-1 (A-12): sc-74445. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining of glandular cells.
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