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- mouse monoclonal IgG1, 200µg/ml
- raised against amino acids 1-300 of NF-1 of human origin
- recommended for detection of all NF-1 isoforms of mouse, rat and human origin by WB, IP, IF and ELISA
- TransCruz reagent for Gel Supershift and ChIP applications, sc-74444 X, 200 µg/0.1 ml
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Ordering Information
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NF-1 Background Information
NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, å-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.
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NF-1 (D-2)
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NF-1 (D-2): sc-74444. Western blot analysis of NF-1 isoform expression in HeLa (A), NIH/3T3 (B) and KNRK (C) nuclear extracts.
NF-1 (D-2): sc-74444. Western blot analysis of NF-1 expression in non-transfected 293T: sc-117752 (A), human NF-1 transfected 293T: sc-159115 (B) and Jurkat (C) whole cell lysates. NF-1 (D-2): sc-74444. Western blot analysis of NF-1 expression in non-transfected: sc-117752 (A) and human NF-1 transfected: sc-159124 (B) 293T whole cell lysates. |
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