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cyclin B1 (D-11) Antibody: sc-7393 |
| Datasheet |
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- mouse monoclonal IgG1; 200 µg/ml
- raised against amino acids 1-433 representing full length cyclin B1 of human origin
- recommended for detection of cyclin B1 of mouse and human origin by WB, IP, IF, IHC(P) and FCM
- agarose conjugate for IP studies, sc-7393 AC, 500 µg/0.25 ml agarose
- phycoerythrin conjugate for intracellular FCM, sc-7393 PE, 100 tests
- Alexa Fluor® 405 (sc-7393 AF405), Alexa Fluor® 488 (sc-7393 AF488) and Alexa Fluor® 647 (sc-7393 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
CCNB1 |
891 |
5q13.2 |
NM_031966 |
P14635
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123836 |
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cyclin B1 Background Information
In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme; tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The specificity of this effect is shown by the inability of either cyclin A or cyclin D1 to display any such stimulation of Cdc25A or Cdc25B.
| cyclin B1 (D-11) Product Citations |
See how others have used cyclin B1 (D-11): sc-7393 antibody and or cyclin B1 (D-11) antibody conjugates.
21 total citations Loading citations.
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cyclin B1 (D-11)
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cyclin B1 (D-11): sc-7393. Western blot analysis of cyclin B1 expression in K-562 (A) and Jurkat (B) nuclear extracts.
cyclin B1 (D-11) PE: sc-7393 PE. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-2866. cyclin B1 (D-11): sc-7393. Western blot analysis of cyclin B1 expression in lysates prepared from untreated (A,C) and etoposide-treated (B,D) HeLa (A,B) and K-562 (C,D) whole cell lysates.
cyclin B1 (D-11) Alexa Fluor 488: sc-7393 AF488. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-3890. cyclin B1 (D-11) Alexa Fluor 647: sc-7393 AF647. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-24636. cyclin B1 (D-11): sc-7393. Western blot analysis of cyclin B1 expression in non-transfected: sc-117752 (A) and mouse cyclin B1 transfected: sc-119543 (B) 293T whole cell lysates and K-562 nuclear extract (C). cyclin B1 (D-11): sc-7393. Western blot analysis of cyclin B1 expression in untreated (A) and Piceatannol ( sc-200610) treated (B) HCT 116 whole cell lysates. Note down regulation of cyclin B1 expression in lane B. cyclin B1 (D-11): sc-7393. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferous ducts.
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