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- mouse monoclonal IgG2a; 200 µg/ml
- specific to epitope mapping within an internal region of the influenza hemagglutinin (HA) protein
- recommended for detection of proteins containing the HA tag by WB, IP, IF, FCM and ELISA
- blocking peptide, sc-7392 P
- agarose conjugate for IP studies, sc-7392 AC, 500 µg/0.25 ml agarose
- Available as TransCruz reagent for ChIP applications, sc-7392 X, 200 µg/0.1 ml
- HRP conjugate, sc-7392 HRP, 200 µg/ml
- biotin conjugate, sc-7392 B, 200 µg/ml
- fluorescein (sc-7392 FITC) conjugate for immunofluorescence, 200 µg/ml, and phycoerythrin (sc-7392 PE), PerCP (sc-7392 PerCP) and PerCP-Cy5.5 (sc-7392 PCPC5) conjugates for FCM, 100 tests
- Alexa Fluor® 405 (sc-7392 AF405), Alexa Fluor® 488 (sc-7392 AF488) and Alexa Fluor® 647 (sc-7392 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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HA-probe Background Information
Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA). The HA epitope tag is useful in Western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-epitope tag.
| HA-probe (F-7) Product Citations |
See how others have used HA-probe (F-7): sc-7392 antibody and or HA-probe (F-7) antibody conjugates.
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HA-probe (F-7)
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HA-probe (F-7): sc-7392. Western blot analysis of HA-tagged fusion proteins showing N-terminal HA-tagged JNK2 (A) and JNK1 (C) and C-terminal HA-tagged Daxx (B).
HA-probe (F-7): sc-7392. Immunofluorescence staining of methanol-fixed COS cells transfected with HA fusion protein showing cytoplasmic staining.
pCruz HA: sc-5045. Western blot analysis of HA-Lac Z fusion protein expression in COS cells transfected with pCruz HA-Lac Z (A) and pCruz His-Lac Z (B). Blot probed with HA-probe (F-7): sc-7392. HA-probe (F-7): sc-7392. Western blot analysis of HA-probe expression in Jurkat whole cell lysate.
HA-probe (F-7): sc-7392. Immunofluorescence staining of methanol-fixed untransfected (A) and HA-probe transfected HEK 293T cells (B).
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