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- mouse monoclonal IgG2a; 200 µg/ml
- epitope corresponding to a short amino acid sequence containing phosphorylated Tyr 204 of ERK 1 of human origin (identical to corresponding ERK2 sequence)
- recommended for detection of ERK 1 phosphorylated at Tyr 204 and correspondingly phosphorylated ERK 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- blocking peptide, sc-7383 P
- agarose conjugate for IP studies, sc-7383 AC, 500 µg/0.25 ml agarose
- fluorescein (sc-7383 FITC) and rhodamine (sc-7383 TRITC) conjugates for immunofluorescence, 200 µg/ml
- for phosphorylation positive (sc-2221) and non-activated control (sc-2200) cell extracts
- phycoerythrin (sc-7383 PE), PerCP (sc-7383 PerCP) and PerCP-Cy5.5 (sc-7383 PCPC5) conjugates for FCM, 100 tests
- Alexa Fluor® 405 (sc-7383 AF405), Alexa Fluor® 488 (sc-7383 AF488) and Alexa Fluor® 647 (sc-7383 AF647) conjugates for immunofluorescence; 100 µg/2 ml
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ERK Background Information
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
| p-ERK (E-4) Product Citations |
See how others have used p-ERK (E-4): sc-7383 antibody and or p-ERK (E-4) antibody conjugates.
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p-ERK (E-4)
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Western blot analysis of ERK activation in untreated (A,C) and UV-irradiated (B,D) HeLa cells. Antibodies tested include a control antibody, ERK 1 (K-23): sc-94 (A,B) and a phospho-ERK-specific antibody, p-ERK (E-4): sc-7383 (C,D). p-ERK (E-4): sc-7383. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization of activated ERK.
p-ERK (E-4): sc-7383. Western blot analysis of ERK 1 phosphorylation in non-transfected 293T: sc-117752 (A), mouse ERK 1 transfected 293T: sc-126806 (B) and A-431 (C) whole cell lysates. p-ERK (E-4): sc-7383. Western blot analysis of ERK phosphorylation in untreated (A), PDGF treated (B) and PDGF and lambda protein phosphatase treated (C) NIH-3T3 whole cell lysates.
Western blot analysis of ERK phosphorylation in untreated (A, C), and lambda protein phosphatase treated (B, D) NIH/3T3 whole cell lysates. Antibodies tested include p-ERK (E-4): sc-7383 (A, B) and ERK 2 (K-23): sc-153 (C, D). p-ERK (E-4): sc-7383. Western blot analysis of ERK phosphorylation in untreated (A) and Raf Kinase Inhibitor V ( sc-222241) treated (B) SK-MEL-28 whole cell lysates. Note inhibition of ERK phosphorylated in lane B. Western blot analysis of ERK phosphorylation in untreated (A,C) and Sorafenib ( sc-220125) treated (B,D) SK-MEL-28 whole cell lysates. Antibodies tested include p-ERK (E-4): sc-7383 (A,B) and ERK 2 (K-23): sc-153 (C,D). Note inhibition of ERK phosphorylation by Sorafenib in lane B. Western blot analysis of ERK phosphorylation in untreated (A,C) and Sorafenib ( sc-220125) treated (B,D) SW480 whole cell lysates. Antibodies tested include p-ERK (E-4): sc-7383 (A,B) and ERK 2 (K-23): sc-153 (C,D). Note inhibition of ERK phosphorylation by Sorafenib in lane B. Western blot analysis of ERK phosphorylation in untreated (A) and L-779,450 ( sc-204046) treated (B) SK-MEL-28 whole cell lysates. Antibody tested is p-ERK (E-4): sc-7383 (A,B). Note inhibition of ERK phosphorylation by L-779,450 in lane B. Western blot analysis of ERK phosphorylation in untreated (A) and L-779,450 ( sc-204046) treated (B) SW480 whole cell lysates. Antibody tested is p-ERK (E-4): sc-7383 (A,B). Note inhibition of ERK phosphorylation by L-779,450 in lane B. Western blot analysis of ERK phosphorylation in untreated (A) and PD 0325901 ( sc-205427) treated (B) SK-MEL-28 whole cell lysates. Antibody tested is p-ERK (E-4): sc-7383 (A,B). Note inhibition of ERK phosphorylation by PD 0325901 in lane B. Western blot analysis of ERK phosphorylation in untreated (A) and PD 0325901 ( sc-205427) treated (B) SW480 whole cell lysates. Antibody tested is p-ERK (E-4): sc-7383 (A,B). Note inhibition of ERK phosphorylation by PD 0325901 in lane B. p-ERK (E-4): sc-7383. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization of activated ERK.
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