raised against TAF II p250 isolated from HeLa cells
produced by immunization with TAF II p250 isolated from HeLa cells
recommended for detection of TAF II p250 of human origin by WB, IP, IF and IHC(P); non cross-reactive with TAF II p250 of mouse, rat, hamster or Drosophila origin
TransCruz reagent for Gel Supershift and ChIP applications, sc-735 X, 200 µg/0.1 ml
TAF II p250 Background Information TFIID is a general transcription factor which initiates pre-initiation complex assembly through direct interaction with the TATA promoter element. It is a multi-subunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs). Although native TFIID can mediate both activator-independent (basal) and activator-dependent transcription in reconstituted systems, TBP can mediate only basal transcription. The largest subunit (TAF) of TFIID is a protein designated TAF II p250. Of interest, TAF II p250 has been cloned and shown to be identical to CCG1, a nuclear DNA-binding protein known to be important for cell cycle progression. This part of TAF II p250 may serve a specific function in activation of a subset of genes important for cell cycle progression.
TAF II p250 (6B3) Product Citations
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TAF II p250 (6B3)
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TAF II p250 (6B3): sc-735. Western blot analysis of hTAF II p250 expression in HeLa (A) and A-431 (B) whole cell lysates.
TAF II p250 (6B3): sc-735. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.
Western blot analysis of TAF II complex in HeLa (A) and A-431 (B) nuclear extracts immunoprecipitated with TAF II p100 (6C1): sc-743 and detected with TAF II p250 (6B3): sc-735.
TAF II p250 (6B3): sc-735. Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear staining of lymphoid cells and squamous epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
