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JNK Antibody (D-2): sc-7345

 |  Datasheet

(Based on data analysis)

  • JNK Antibody (D-2) is a mouse monoclonal IgG1 provided at 200 µg/ml
  • raised against amino acids 1-424 representing full length JNK of human origin
  • recommended for detection of all JNK1, JNK2 and JNK3 p46 and p54 isoforms of mouse, rat and human by WB, IP, IF, IHC(P), FCM and ELISA
  • agarose conjugate for IP studies, sc-7345 AC, 500 µg/0.25 ml agarose
  • phycoerythrin (sc-7345 PE) conjugate for intracellular FCM, 100 tests
  • HRP conjugate, sc-7345 HRP, 200 µg/ml
  • Alexa Fluor® 405 (sc-7345 AF405), Alexa Fluor® 488 (sc-7345 AF488) and Alexa Fluor® 647 (sc-7345 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
  • See 77 citations for JNK Antibody (D-2)
 
Full-length JNK protein sequence with immunogen highlighted:
MSDSKCDSQFYSVQVADSTFTVLKRYQQLKPIGSGAQGIVCAAFDTVLGISVAVKKLSRPFQNQTHAKRAYRELVLLKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIHMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTACTNFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGELVKGCVIFQGTDHIDQWNKVIEQLGTPSAEFMKKLQPTVRNYVENRPKYPGIKFEELFPDWIFPSESERDKIKTSQARDLLSKMLVIDPDKRISVDEALRHPYITVWYDPAEAEAPPPQIYDAQLEEREHAIEEWKELIYKEVMDWEERSKNGVVKDQPSDAAVSSNATPSQSSSINDISSMSTEQTLASDTDSSLDASTGPLEGC

See additional JNK Antibodies including JNK, JNK1, JNK2 and JNK3.

Also see chemical JNK Inhibitors or chemical JNK Activators for functional analysis of cellular responses to JNK.


Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   FCM  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
JNK Antibody (D-2) sc-7345 200 µg/ml $279
JNK Antibody (D-2) AC sc-7345 AC 500 µg/ml, 25% ag $366
JNK Antibody (D-2) Alexa Fluor® 405 sc-7345 AF405 100 µg/2 ml $314
JNK Antibody (D-2) Alexa Fluor® 488 sc-7345 AF488 100 µg/2 ml $314
JNK Antibody (D-2) Alexa Fluor® 647 sc-7345 AF647 100 µg/2 ml $314
JNK Antibody (D-2) HRP sc-7345 HRP 200 µg/ml $279
JNK Antibody (D-2) PE sc-7345 PE 100 tests in 2 ml $303
 CRISPR/Cas9 KO and HDR Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
JNK1 CRISPR/Cas9 KO Plasmid (h) sc-400048 20 µg $345
JNK1 HDR Plasmid (h) sc-400048-HDR 20 µg $385
JNK2 CRISPR/Cas9 KO Plasmid (h) sc-400115 20 µg $345
JNK2 HDR Plasmid (h) sc-400115-HDR 20 µg $385
JNK3 CRISPR/Cas9 KO Plasmid (h) sc-400236 20 µg $345
JNK3 HDR Plasmid (h) sc-400236-HDR 20 µg $385
JNK3 CRISPR/Cas9 KO Plasmid (m) sc-424049 20 µg $345
JNK3 HDR Plasmid (m) sc-424049-HDR 20 µg $385
JNK1 CRISPR/Cas9 KO Plasmid (m) sc-424053 20 µg $345
JNK1 HDR Plasmid (m) sc-424053-HDR 20 µg $385
JNK2 CRISPR/Cas9 KO Plasmid (m) sc-424054 20 µg $345
JNK2 HDR Plasmid (m) sc-424054-HDR 20 µg $385
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
mouse brain extract sc-2253 500 µg/200 µl $104
JNK2 (m): 293T Lysate sc-125507 100 µg/200 µl $205
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
JNK2 (h): 293T Lysate sc-115269 100 µg/200 µl $205
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
A-431 Whole Cell Lysate sc-2201 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
 WB Control Recombinant Protein (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
JNK2 (1-424) sc-4062 50 µg $167
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human MAPK9 5601 5q35.3 NM_002752, NM_139068, NM_139069, NM_139070 P45984
602896
Mouse Mapk10 26414 5 E5 Q61831
N/A
Mouse Mapk8 26419 14 B Q91Y86
N/A
Mouse Mapk9 26420 11 B1.2 Q9WTU6
N/A
 


c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1a1,JNK1b1, JNK2a1, JNK2b1, and JNK3a1, which represent the p46 isoforms, and JNK1a2, JNK1b2, JNK2a2, JNK2b2, and JNK3b2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun, and other signaling kinases, including MEKK1 and p38.

JNK Antibody (D-2) Product Citations
See how others have used JNK Antibody (D-2) and or JNK Antibody (D-2) conjugates.


77 total citations
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JNK Antibody (D-2) Data
Click on image to enlarge
JNK (D-2): sc-7345. Western blot analysis of JNK expression in NIH/3T3 whole cell lysate.
JNK (D-2): sc-7345. Immunofluorescence staining of methanol-fixed HeLa cells showing JNK localization.
JNK (D-2): sc-7345. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.
JNK (D-2) Alexa Fluor 488: sc-7345 AF488. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-3890.
JNK (D-2) PE: sc-7345 PE. Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-2866.histogram represents control mouse IgG1.
JNK (D-2): sc-7345. Western blot analysis of JNK2 expression in non-transfected: sc-117752 (A) and mouse JNK2 transfected: sc-125507 (B) 293T whole cell lysates.
Western blot analysis of JNK phosphorylation in untreated (A,D), UV treated (B,E) and UV and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-JNK (Thr 183/Tyr 185): sc-12882 (A,B,C) and JNK (D-2): sc-7345 (D,E,F).
JNK (D-2): sc-7345. Western blot analysis of JNK2 expression in non-transfected: sc-117752 (A) and human JNK2 transfected: sc-115269 (B) 293T whole cell lysates.
Western blot analysis of JNK phosphorylation in untreated (A,D), anisomycin treated (B,E) and anisomycin and lambda protein phosphatase (sc-200312A) treated (C,F) NIH/3T3 whole cell lysates. Antibodies tested include p-JNK (Thr 183/Tyr 185): sc-12882-R (A,B,C) and JNK (D-2): sc-7345 (D,E,F).
JNK (D-2): sc-7345. Western blot analysis of JNK2 expression in non-transfected 293T: sc-117752 (A), human JNK2 transfected 293T: sc-158651 (B) and K-562 (C) whole cell lysates.
JNK (D-2): sc-7345. Western blot analysis of JNK2 expression in non-transfected: sc-117752 (A) and human JNK2 transfected: sc-158651 (B) 293T whole cell lysates.
Western blot analysis of JNK phosphorylation in untreated (A,D), anisomycin treated (B,E) and anisomycin and lambda protein phosphatase (sc-200312A) treated (C,F) NIH/3T3 whole cell lysates. Antibodies tested include p-JNK (G-7): sc-6254 (A,B,C) and JNK (D-2): sc-7345 (D,E,F).
Western blot analysis of JNK phosphorylation in untreated (A,D), UV treated (B,E) and UV and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-JNK (G-7): sc-6254 (A,B,C) and JNK (D-2): sc-7345 (D,E,F).
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