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home » Kinases and Phosphatases » JNK Antibody / JNK Antibodies » JNK (D-2): sc-7345

JNK (D-2) Antibody: sc-7345

  |  Datasheet
  • mouse monoclonal IgG1; 200 µg/ml
  • raised against amino acids 1-424 representing full length JNK of human origin
  • recommended for detection of all JNK1, JNK2 and JNK3 p46 and p54 isoforms of mouse, rat and human by WB, IP, IF, IHC(P), FCM and ELISA
  • agarose conjugate for IP studies, sc-7345 AC, 500 µg/0.25 ml agarose
  • phycoerythrin (sc-7345 PE) conjugate for intracellular FCM, 100 tests
  • HRP conjugate, sc-7345 HRP, 200 µg/ml
  • Alexa Fluor® 405 (sc-7345 AF405), Alexa Fluor® 488 (sc-7345 AF488) and Alexa Fluor® 647 (sc-7345 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
 
Additional JNK Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   FCM  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human MAPK9 5601 5q35.3 NM_002752, NM_139068, NM_139069, NM_139070 P45984
 602896
Mouse Mapk10 26414 5 E5 Q61831
 N/A
Mouse Mapk8 26419 14 B Q91Y86
 N/A
Mouse Mapk9 26420 11 B1.2 Q9WTU6
 N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
JNK (D-2) sc-7345 200 µg/ml $279
JNK (D-2) AC sc-7345 AC 500µg/ml, 25%ag $366
JNK (D-2) Alexa Fluor® 405 sc-7345 AF405 100 µg/2 ml $314
JNK (D-2) Alexa Fluor® 488 sc-7345 AF488 100 µg/2 ml $314
JNK (D-2) Alexa Fluor® 647 sc-7345 AF647 100 µg/2 ml $314
JNK (D-2) HRP sc-7345 HRP 200 µg/ml $279
JNK (D-2) PE sc-7345 PE 100 tests in 2ml $303
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
mouse brain extract sc-2253 500 µg/200 µl $104
JNK2 (m): 293T Lysate sc-125507 100µg/200µl $205
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
JNK2 (h): 293T Lysate sc-115269 100µg/200µl $205
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
A-431 Whole Cell Lysate sc-2201 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
 WB Control Recombinant Protein (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
JNK2 (1-424) sc-4062 50 µg $167

JNK Background Information
c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1a1,JNK1b1, JNK2a1, JNK2b1, and JNK3a1, which represent the p46 isoforms, and JNK1a2, JNK1b2, JNK2a2, JNK2b2, and JNK3b2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun, and other signaling kinases, including MEKK1 and p38.

JNK (D-2) Product Citations
See how others have used JNK (D-2): sc-7345 antibody and or JNK (D-2) antibody conjugates.


50 total citations
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Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
 
JNK (D-2)
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sc-7345
JNK (D-2): sc-7345. Immunofluorescence staining of methanol-fixed HeLa cells showing JNK localization (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining (B).
sc-7345
JNK (D-2): sc-7345. Western blot analysis of JNK expression in NIH/3T3 whole cell lysate.
sc-7345
JNK (D-2) PE: sc-7345 PE. Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-2866.
sc-7345
JNK (D-2) Alexa Fluor 488: sc-7345 AF488. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-3890.
sc-7345
JNK (D-2): sc-7345. Western blot analysis of JNK2 expression in non-transfected: sc-117752 (A) and mouse JNK2 transfected: sc-125507 (B) 293T whole cell lysates.
sc-7345
Western blot analysis of JNK phosphorylation in untreated (A,D), UV treated (B,E) and UV and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-JNK (Thr 183/Tyr 185): sc-12882 (A,B,C) and JNK (D-2): sc-7345 (D,E,F).
sc-7345
JNK (D-2): sc-7345. Western blot analysis of JNK2 expression in non-transfected: sc-117752 (A) and human JNK2 transfected: sc-115269 (B) 293T whole cell lysates.
sc-7345
Western blot analysis of JNK phosphorylation in untreated (A,D), anisomycin treated (B,E) and anisomycin and lambda protein phosphatase (sc-200312A) treated (C,F) NIH/3T3 whole cell lysates. Antibodies tested include p-JNK (Thr 183/Tyr 185): sc-12882-R (A,B,C) and JNK (D-2): sc-7345 (D,E,F).
sc-7345
JNK (D-2): sc-7345. Western blot analysis of JNK2 expression in non-transfected 293T: sc-117752 (A), human JNK2 transfected 293T: sc-158651 (B) and K-562 (C) whole cell lysates.
sc-7345
JNK (D-2): sc-7345. Western blot analysis of JNK2 expression in non-transfected: sc-117752 (A) and human JNK2 transfected: sc-158651 (B) 293T whole cell lysates.
sc-7345
Western blot analysis of JNK phosphorylation in untreated (A,D), anisomycin treated (B,E) and anisomycin and lambda protein phosphatase (sc-200312A) treated (C,F) NIH/3T3 whole cell lysates. Antibodies tested include p-JNK (G-7): sc-6254 (A,B,C) and JNK (D-2): sc-7345 (D,E,F).
sc-7345
Western blot analysis of JNK phosphorylation in untreated (A,D), UV treated (B,E) and UV and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-JNK (G-7): sc-6254 (A,B,C) and JNK (D-2): sc-7345 (D,E,F).
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